Direct electrochemistry of glucose oxidase on a graphite nanosheet-Nafion composite film modified electrode

被引:111
作者
Fu, Chenglin [1 ]
Yang, Wensheng [1 ]
Chen, Xu [1 ]
Evans, David G. [1 ]
机构
[1] Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China
基金
中国国家自然科学基金;
关键词
Graphite nanosheets; Nafion; Direct electrochemistry; Glucose oxidase; Biosensor; CARBON NANOTUBES; ENZYMATIC-ACTIVITY; GLASSY-CARBON; NANOPARTICLE; BIOSENSORS; ENZYMES; NADH;
D O I
10.1016/j.elecom.2009.02.042
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
The direct electrochemistry of glucose oxidase (GOD) immobilized in a modified electrode based on a composite film of exfoliated graphite nanosheets (GNSs) and Nafion has been investigated for the first time. Direct electron communication between GOD and the electrode was achieved with a fast electron transfer rate (12.6 s(-1)). In addition, the bioactivity of GOD was retained after immobilization in the composite film and glucose could be determined based on the decrease of the electrocatalytic response of the reduced form of GOD to dissolved oxygen. The resulting biosensor exhibited higher sensitivity (3.4 mu A mM(-1)). Considering much lower cost of GNSs and ready preparation from graphite, the GNSs-based modified electrode described here is superior to the carbon nanotubes (CNTs)-based modified electrodes and should have wide applications in third-generation biosensors, bioelectronics and electrocatalysis. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:997 / 1000
页数:4
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