Automated routine HLA-B27 typing by flow cytometry

被引：9

作者：

Reynolds, WM

Evans, PR

Wilson, PJ

Wong, WM

Darke, C

Smith, JL

机构：

[1] SOUTHAMPTON UNIV NHS HOSP TRUST,WESSEX IMMUNOL SERV,WESSEX HISTOCOMPATIBIL GRP,SOUTHAMPTON SO16 6YD,HANTS,ENGLAND

[2] SOUTHAMPTON UNIV NHS HOSP TRUST,DEPT RHEUMATOL,SOUTHAMPTON SO16 6YD,HANTS,ENGLAND

[3] NATL BLOOD TRANSFURS SERV WALES,REG TISSUE TYPING LAB,CARDIFF CF5 6XF,S GLAM,WALES

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1996年 / 197卷 / 1-2期

关键词：

HLA-B27; flow cytometry; monoclonal antibody; ankylosing spondylitis;

D O I：

10.1016/0022-1759(96)00158-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An increasing demand for HLA-B27 typing as one of the tests used in the diagnosis of ankylosing spondylitis has led us to develop a rapid, automated, flow cytometric assay using whole blood and an HLA-B27 specific monoclonal antibody FD705. This article shows the data from 2093 samples tested during a 2 year period of routine HLA-B27 typing. 21.6% were clearly HLA-B27 positive whilst 73.2% were HLA-B27 negative, the remaining 5.2% required further testing before assignment of HLA-B27 status. Additional work was carried out on blood samples from individuals positive for the newly described subtype HLA-B2708.

引用

页码：1 / 5

页数：5

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