Purification and characterization of a corrinoid compound from Chlorella tablets as an algal health food

Vitamin B-12 content of an algal health food, Chlorella tablets (Chlorella sp.), was determined by both Lactobacillus leichmannii ATCC 7830 microbiological and intrinsic factor-chemiluminescence methods. The values of 200.9-211.6 mug/100 g dry weight determined by the chemiluminescence method were similar to the values (201.3-285.7 mug/100 g dry weight) determined by the microbiological method. A corrinoid compound was purified to homogeneity from the Chlorella tablets and characterized. The purified corrinoid compound was identified as vitamin 1312, on the basis of silica gel 60 TLC, C18 reversed-phase HPLC, H-1 NMR spectroscopy, and UV-Vis spectroscopy.