Solubilizing coelenterazine in water with hydroxypropyl-beta-cyclodextrin

被引：14

作者：

Teranishi, K ^{[1
]}

Shimomura, O ^{[1
]}

机构：

[1] MARINE BIOL LAB, WOODS HOLE, MA 02543 USA

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1997年 / 61卷 / 07期

关键词：

coelenterazine; hydroxypropyl-beta-cyclodextrin; solubility;

D O I：

10.1271/bbb.61.1219

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Coelenterazine is poorly soluble in water except at alkaline pH values that promote auto-oxidation. The solubility is markedly increased by adding hydroxypropyl-beta-cyclodextrin in a neutral buffer solution, the solubilization being most effective in the pH range of 6.5 to 7.0. The concentration of coelenterazine dissolved in a pH 7.0 buffer containing 50 mM hydroxypropyl-beta-cyclodextrin was about 280-fold higher than that without the additive.

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收藏

页码：1219 / 1220

页数：2

相关论文

共 19 条

[1]

Blinks J.R., 1978, Methods in Enzymology, V57, P292

[2] TRANSFECTED AEQUORIN IN THE MEASUREMENT OF CYTOSOLIC CA2+ CONCENTRATION ([CA2+](C)) - A CRITICAL-EVALUATION [J].

BRINI, M ;

MARSAULT, R ;

BASTIANUTTO, C ;

ALVAREZ, J ;

POZZAN, T ;

RIZZUTO, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (17) :9896-9903

[3] NUCLEAR TARGETING OF AEQUORIN - A NEW APPROACH FOR MEASURING NUCLEAR CA2+ CONCENTRATION IN INTACT-CELLS [J].

BRINI, M ;

MARSAULT, R ;

BASTIANUTTO, C ;

POZZAN, T ;

RIZZUTO, R .

CELL CALCIUM, 1994, 16 (04) :259-268

[4]

GOTO T, 1980, MARINE NATURAL PRODU, P179

[5] REVISION OF THE STRUCTURE OF THE LIGHT-EMITTER IN AEQUORIN BIOLUMINESCENCE [J].

HIRANO, T ;

MIZOGUCHI, I ;

YAMAGUCHI, M ;

CHEN, FQ ;

OHASHI, M ;

OHMIYA, Y ;

TSUJI, FI .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1994, (02) :165-167

[6] CHEMILUMINESCENCE OF RENILLA (SEA PANSY) LUCIFERIN AND ITS ANALOGS [J].

HORI, K ;

WAMPLER, JE ;

CORMIER, MJ .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1973, (14) :492-493

[7] BIOLUMINESCENCE OF THE CA-2+-BINDING PHOTOPROTEIN AEQUORIN AFTER CYSTEINE MODIFICATION [J].

KUROSE, K ;

INOUYE, S ;

SAKAKI, Y ;

TSUJI, FI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (01) :80-84

[8] MONITORING DYNAMIC CHANGES IN FREE CA2+ CONCENTRATION IN THE ENDOPLASMIC-RETICULUM OF INTACT-CELLS [J].

MONTERO, M ;

BRINI, M ;

MARSAULT, R ;

ALVAREZ, J ;

SITIA, R ;

POZZAN, T ;

RIZZUTO, R .

EMBO JOURNAL, 1995, 14 (22) :5467-5475

[9] STRUCTURE OF THE FUNCTIONAL PART OF PHOTOPROTEIN AEQUORIN [J].

MUSICKI, B ;

KISHI, Y ;

SHIMOMURA, O .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1986, (21) :1566-1568

[10] GALACTOSE-DEPENDENT EXPRESSION OF THE RECOMBINANT CA-2+-BINDING PHOTOPROTEIN AEQUORIN IN YEAST [J].

NAKAJIMASHIMADA, J ;

IIDA, H ;

TSUJI, FI ;

ANRAKU, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 174 (01) :115-122