Analysis of an adenine nucleotide-containing metabolite of clodronate using ion pair high-performance liquid chromatography-electrospray ionisation mass spectrometry

被引：35

作者：

Mönkkönen, H

Moilanen, P

Mönkkönen, J

Frith, JC

Rogers, MJ

Auriola, S

机构：

[1] Univ Kuopio, Dept Pharmaceut, FIN-70211 Kuopio, Finland

[2] Univ Kuopio, Dept Pharmaceut Chem, FIN-70211 Kuopio, Finland

[3] Univ Aberdeen, Dept Med & Therapeut, Aberdeen, Scotland

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2000年 / 738卷 / 02期

关键词：

clodronate;

D O I：

10.1016/S0378-4347(99)00559-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Clodronate belongs to the family of bisphosphonates, which are synthetic analogues of pyrophosphate. Bisphosphonates are widely used in the treatment of metabolic bone diseases. Some bisphosphonates, including clodronate, can be metabolized in cells into non-hydrolysable nucleotide analogues. In this paper. we describe a new method for extraction and quantitation of the clodronate metabolite in cell lysates by using ion-pairing HPLC method that is compatible with negative ion electrospray ionization mass spectrometry (ESI-MS). The method was used for detection of the metabolite of clodronate in extracts from RAW 264 macrophage cells after treatment with clodronate. (C) 2000 Elsevier Science B.V. All rights reserved.

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收藏

页码：395 / 403

页数：9

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