Synthesis and Characterization of a Carbon Nanotube-Dendron Series for Efficient siRNA Delivery

被引：139

作者：

Antonia Herrero, M. ^{[2
,3
,5
]}

Toma, Francesca M. ^{[2
,3
,4
]}

Al-Jamal, Khuloud T. ^{[1
]}

Kostarelos, Kostas ^{[1
]}

Bianco, Alberto ^{[6
]}

Da Ros, Tatiana ^{[2
,3
]}

Bano, Fouzia ^{[4
,7
]}

Casalis, Loredana ^{[4
,7
]}

Scoles, Giacinto ^{[4
,7
]}

Prato, Maurizio ^{[2
,3
]}

机构：

[1] Univ London, Sch Pharm, Nanomed Lab, Ctr Drug Delivery Res, London WC1N 1AX, England

[2] Univ Trieste, Ctr Excellence Nanostruct Mat, Dept Pharmaceut Sci, I-34127 Trieste, Italy

[3] Univ Trieste, INSTM, I-34127 Trieste, Italy

[4] SISSA, I-34014 Trieste, Italy

[5] Univ Castilla La Mancha, Fac Quim, Dept Quim Organ, E-13071 Ciudad Real, Spain

[6] CNRS, Inst Biol Mol & Cellulaire, UPR Immunol & Chim Therapeut 9021, F-67000 Strasbourg, France

[7] Elettra, Synchrotron Labs, I-34149 Trieste, Italy

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 28期

关键词：

SMALL INTERFERING RNA; INTRACELLULAR DELIVERY; FUNCTIONALIZATION; CELLS; GENE; BIODISTRIBUTION; IMMOBILIZATION; TRANSLOCATION; TRANSPORTERS; TOXICOLOGY;

D O I：

10.1021/ja903316z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new series of dendron-functionalized multiwalled carbon nanotube (MWNT) derivatives, characterized by the presence of numerous positively charged tetraalkyl ammonium salts at the periphery of the dendron, has been synthesized. The positive charges on the MWNT surface, coupled with the unique ability of carbon nanotubes (CNTs) to penetrate cell membranes, make the new derivatives potentially ideal vectors for siRNA delivery. Using a fluorescently labeled, noncoding siRNA sequence, we demonstrate that cytoplasmic delivery of the nucleic acid is remarkably increased throughout the different dendron generations. The work reported here highlights the fact that dendron-functionalized CNTs can be rationally designed as efficient carriers of siRNA that can eventually lead to gene silencing.

引用

页码：9843 / 9848

页数：6

共 44 条

[1] Multiwalled carbon nanotube-doxorubicin supramolecular complexes for cancer therapeutics [J].