New set of microsatellite loci isolated in apricot

被引：94

作者：

Messina, R ^{[1
]}

Lain, O ^{[1
]}

Marrazzo, MT ^{[1
]}

Cipriani, G ^{[1
]}

Testolin, R ^{[1
]}

机构：

[1] Univ Udine, Dipartimento Sci Agrarie & Ambientali, I-33100 Udine, Italy

来源：

MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 03期

关键词：

apricot; fingerprinting; molecular markers; Prunus; simple sequence repeat;

D O I：

10.1111/j.1471-8286.2004.00674.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report 99 simple sequence repeats (SSRs) newly isolated from an apricot (Prunus armeniaca L.) genomic library enriched for AG/CT repeats. Twenty SSRs were screened for their polymorphism in 16 apricot cultivars. The number of alleles ranged from two to nine, whereas the expected heterozygosity (H-E) ranged from 0.26 to 0.82. The same SSRs showed also an appreciable transportability across different Prunus species, such as peach, nectarine, almond, European plum, Japanese plum, sweet cherry and sour cherry, with 20% of primers giving successful amplifications in all Prunus species assayed. None gave amplification in apple.

引用

页码：432 / 434

页数：3

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