Latest developments and in vivo use of the Tet system:: ex vivo and in vivo delivery of tetracycline-regulated genes

被引：65

作者：

Corbel, SY ^{[1
]}

Rossi, FM ^{[1
]}

机构：

[1] Univ British Columbia, Biomed Res Ctr, Vancouver, BC V6T 1Z3, Canada

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2002年 / 13卷 / 05期

关键词：

D O I：

10.1016/S0958-1669(02)00361-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In June this year, the tetracycline-regulated gene expression system (tet system) celebrated its tenth 'birthday'. In the past ten years a continuous stream of changes made to the tet system's basic components has led to a remarkable improvement in its overall performance. It was not until this year, however, that the full benefits of these improvements became apparent. In particular, usage of the tet system is no longer limited to immortalized cell lines and transgenic animals. In this review, we will describe the obstacles encountered in delivering the tet system's components to primary cells and tissues as well as the methods now used to overcome them. We will also focus on a novel system that is conceptually similar but based on different antibiotic/transcription factor pairs.

引用

页码：448 / 452

页数：5

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