Snr, new genetic loci common to the nitrate reduction systems of Pseudomonas aeruginosa PAO1

被引：3

作者：

Irani, VR ^{[1
]}

Darzins, A ^{[1
]}

Rowe, JJ ^{[1
]}

机构：

[1] UNIV DAYTON, DEPT BIOL, DAYTON, OH 45469 USA

来源：

CURRENT MICROBIOLOGY | 1997年 / 35卷 / 01期

关键词：

D O I：

10.1007/s002849900202

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Pseudomonas aeruginosa is able to both assimilate and dissimilate nitrate. On the basis of the characteristics of mutants unable to dissimilate or assimilate nitrate to nitrite, it was revealed that two different sets of genes (represented by Class I and Class II mutants) were shared between the nitrate-to-nitrite reduction steps of both pathways. The genes represented by Class I and II mutants have been separated into distinct genetic loci using two cosmids, pAD1695/96. The two different genetic loci have been designated snr (shared citrate reduction) and mol (MoCo processing genes) based on the phenotypic characteristics of the mutants complemented. Restriction analyses of pAD1695/96 followed by subcloning confirmed the complementation results. The snr loci, which represent a unique and hitherto uninvestigated set of genes for nitrate reduction, were mapped on the P. aeruginosa chromosome by linkage analysis with sex factor FP2.

引用

页码：9 / 13

页数：5

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