Optimal immobilization of β-galactosidase from Pea (PsBGAL) onto Sephadex and chitosan beads using response surface methodology and its applications

被引：78

作者：

Dwevedi, Alka ^{[1
]}

Kayastha, Arvind M. ^{[1
]}

机构：

[1] Banaras Hindu Univ, Sch Biotechnol, Fac Sci, Varanasi 221005, Uttar Pradesh, India

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 10期

关键词：

beta-Galactosidase; Sephadex; Chitosan; Immobilization; Lactose hydrolysis; PACKED-BED REACTOR; LACTOSE HYDROLYSIS; IN-VITRO; OPTIMIZATION; STABILITY; BACTERIA; ENZYMES; UREASE; SYSTEM; MEDIA;

D O I：

10.1016/j.biortech.2008.12.048

中图分类号：

S2 [农业工程];

学科分类号：

082806 [农业信息与电气工程];

摘要：

Response surface methodology (RSM) and centre composite design (CCD) were used to optimize immobilization of beta-galactosidase (BGAL) from Pisum sativum onto two matrices: Sephadex G-75 and chitosan beads. The immobilization efficiency of 75.66% and 75.19% were achieved with Sephadex G-75 and chitosan, respectively. There was broad divergence in physico-chemical properties of Sephadex-PsBGAL and chitosan-PsBGAL Chitosan-PsBGAL was better suited for industrial application based on its broad pH and temperature optima, higher temperature stability, reusability etc. Sephadex-PsBGAL and chitosan-PsBGAL showed much variation in their catalytic properties with respect to soluble enzyme. About 50% loss in activity of Sephadex-PsBGAL and chitosan-PsBGAL were observed after 12 and 46 days at 4 degrees C, respectively. Chitosan-PsBGAL showed higher rate of lactose hydrolysis present in milk and whey at room temperature and 4 degrees C than Sephadex-PsBGAL. In both cases, lactose of milk whey was hydrolyzed at higher rate than that of milk. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：2667 / 2675

页数：9

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