Metabolism of endothelin-1 by neuroblastoma x glioma hybrid (NG108-15) cells

被引：1

作者：

Angelova, K ^{[1
]}

Ergul, A ^{[1
]}

Peng, KC ^{[1
]}

Puett, D ^{[1
]}

机构：

[1] UNIV GEORGIA,DEPT BIOCHEM & MOL BIOL,ATHENS,GA 30602

来源：

NEUROSCIENCE LETTERS | 1997年 / 225卷 / 01期

关键词：

endothelin-1; NG108-15; cells; endothelin degradation;

D O I：

10.1016/S0304-3940(97)00166-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The endothelin (ET) peptides, ET-1, ET-2, and ET-3, as well as the ETA and ETB receptor subtypes, are known to occur in brain, but there is a dearth of information on the metabolism of these peptides by the central nervous system (CNS). Tn this study we have investigated the kinetics of ET-1 binding to and dissociation from the hybrid neuroblastoma x glioma cell line, NG108-15, which is known to contain functional ET receptors, and metabolism of bound ET-1. [I-125]ET-1 was incubated with cells for various periods of time up to 6 h, and the nature of the radioactivity in the cell medium and lysate was analyzed by reverse phase high performance liquid chromatography (HPLC). It was found that NG108-15 cells are capable of degrading [I-125]ET-1 to [I-125]Tyr and several fragments of intermediate hydrophobicity; however, a portion of the cell-associated [I-125]ET-1 was protected from degradation for several hours. (C) 1997 Elsevier Science Ireland Ltd.

引用

页码：1 / 4

页数：4

共 16 条

[1]

Angelova K, 1996, CELL MOL BIOL, V42, P1243

[2]

Angelova K, 1996, MOL CELL BIOCHEM, V159, P65

[3] Endothelin degradation by vascular smooth muscle cells [J].