Characterization and thermodynamic properties of quadruplex/duplex competition

被引：154

作者：

Li, W

Wu, P

Ohmichi, T

Sugimoto, N

机构：

[1] Konan Univ, High Technol Res Ctr, Higashinada Ku, Kobe, Hyogo 6588501, Japan

[2] Konan Univ, Fac Sci & Engn, Dept Chem, Higashinada Ku, Kobe, Hyogo 6588501, Japan

来源：

FEBS LETTERS | 2002年 / 526卷 / 1-3期

关键词：

G-quadruplex; I-motif; structure competition; duplex; polycation;

D O I：

10.1016/S0014-5793(02)03118-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Structural characteristics and thermodynamic properties of dG(3)(T(2)AG(3))(3), d(C(3)TA(2))(3)C-3 and dG(3)(T(2)AG(3))(3)/d(C(3)TA(2))(3)C-3 were intensively investigated. It was indicated that metal ions greatly affected the conformation and stability of the G-quadruplex. A competition of a structure transition among the G-quadruplex, I-motif, and the duplex was confirmed to be dependent on both cation species and pH values. The structural competitive mechanism is discussed for the first time. This study shows an intriguing potential in modulating DNA structures in vivo, which is of great importance in drug design and cancer chemotherapy. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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页码：77 / 81

页数：5

相关论文

共 27 条

[1] HAIRPIN AND PARALLEL QUARTET STRUCTURES FOR TELOMERIC SEQUENCES [J].

BALAGURUMOORTHY, P ;

BRAHMACHARI, SK ;

MOHANTY, D ;

BANSAL, M ;

SASISEKHARAN, V .

NUCLEIC ACIDS RESEARCH, 1992, 20 (15) :4061-4067

[2]

BALAGURUMOORTHY P, 1994, J BIOL CHEM, V269, P21858

[3] TELOMERES - NO END IN SIGHT [J].

BLACKBURN, EH .

CELL, 1994, 77 (05) :621-623

[4] Structural analysis of G-DNA in solution: A combination of polarized and depolarized dynamic light scattering with hydrodynamic model calculations [J].

Bolten, M ;

Niermann, M ;

Eimer, W .

BIOCHEMISTRY, 1999, 38 (38) :12416-12423

[5] DUPLEX TO QUADRUPLEX EQUILIBRIUM OF THE SELF-COMPLEMENTARY OLIGONUCLEOTIDE D(GGGGCCCC) [J].

DENG, H ;

BRAUNLIN, WH .

BIOPOLYMERS, 1995, 35 (06) :677-681

[6] TELOMERIC DNA OLIGONUCLEOTIDES FORM NOVEL INTRAMOLECULAR STRUCTURES CONTAINING GUANINE GUANINE BASE-PAIRS [J].

HENDERSON, E ;

HARDIN, CC ;

WALK, SK ;

TINOCO, I ;

BLACKBURN, EH .

CELL, 1987, 51 (06) :899-908

[7] Effects of polyamines on the thermal stability and formation kinetics of DNA duplexes with abnormal structure [J].

Hou, MH ;

Lin, SB ;

Yuann, JMP ;

Lin, WC ;

Wang, AHJ ;

Kan, LS .

NUCLEIC ACIDS RESEARCH, 2001, 29 (24) :5121-5128

[8] STABLE LOOP IN THE CRYSTAL-STRUCTURE OF THE INTERCALATED 4-STRANDED CYTOSINE-RICH METAZOAN TELOMERE [J].

KANG, CH ;

BERGER, I ;

LOCKSHIN, C ;

RATLIFF, R ;

MOYZIS, R ;

RICH, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (09) :3874-3878

[9] INTRAMOLECULAR FOLDING OF A FRAGMENT OF THE CYTOSINE-RICH STRAND OF TELOMERIC DNA INTO AN I-MOTIF [J].

LEROY, JL ;

GUERON, M ;

MERGNY, JL ;

HELENE, C .

NUCLEIC ACIDS RESEARCH, 1994, 22 (09) :1600-1606

[10] EVIDENCE FOR INTRAMOLECULARLY FOLDED I-DNA STRUCTURES IN BIOLOGICALLY RELEVANT CCC-REPEAT SEQUENCES [J].

MANZINI, G ;

YATHINDRA, N ;

XODO, LE .

NUCLEIC ACIDS RESEARCH, 1994, 22 (22) :4634-4640