Voltammetric measurement of microorganism populations

被引:27
作者
Ha, SB
Li, X
Guo, GM
Sun, YS
Yuan, ZB [1 ]
机构
[1] Chinese Acad Sci, Univ Sci & Technol China, Grad Sch, Beijing 100039, Peoples R China
[2] Hebei Univ Sci & Technol, Dept Environm Engn, Shijiazhuang 050018, Peoples R China
关键词
biosensor; tetracycline-modified electrode; measurement of microorganism populations; food; environmental;
D O I
10.1016/S0003-2670(99)00753-9
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A voltammetric biosensor was developed for the assay of microorganism populations. A tetracycline-modified glassy carbon electrode was used in the construction of the biosensor and an electrochemical cell with an elastic base was employed in the measurement. The possible mechanism of the electrode response was discussed. The determination limit of the microbial populations was 2-5 x 10(4) cells with cyclic voltammetry and the measuring period for a sample was 30 min. Measurements on 16 samples showed that the method was a rapid and practical way for the determination of microorganism populations in food and environmental samples. (C)2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:115 / 121
页数:7
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