Subgingival microbiota dysbiosis in systemic lupus erythematosus: association with periodontal status

被引:123
作者
Correa, Joice Dias [1 ]
Calderaro, Debora Cerqueira [2 ]
Ferreira, Gilda Aparecida [2 ]
Souza Mendonca, Santuza Maria [1 ]
Fernandes, Gabriel R. [3 ]
Xiao, E. [4 ]
Teixeira, Antonio Lucio [2 ]
Leys, Eugene J. [5 ]
Graves, Dana T. [4 ]
Silva, Tarcilia Aparecida [1 ,6 ]
机构
[1] Univ Fed Minas Gerais, Fac Dent, Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, Univ Hosp, Belo Horizonte, MG, Brazil
[3] Fundacao Oswaldo Cruz, Rene Rachou Res Ctr, Belo Horizonte, MG, Brazil
[4] Univ Penn, Penn Dent Sch, Philadelphia, PA 19104 USA
[5] Ohio State Univ, Coll Dent, Columbus, OH 43210 USA
[6] Univ Fed Minas Gerais, Fac Odontol, Dept Patol Cirurgia Odontol, Av Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG, Brazil
关键词
Lupus; Periodontitis; Oral microbiota; Cytokine; Subgingival dental plaque; Illumina sequencing; RHEUMATOID-ARTHRITIS; PORPHYROMONAS-GINGIVALIS; ORAL MICROBIOTA; GUT MICROBIOTA; DISEASE; HEALTH; RISK; PATHOGENESIS; EXPRESSION; IL-33;
D O I
10.1186/s40168-017-0252-z
中图分类号
Q93 [微生物学];
学科分类号
071005 [微生物学];
摘要
Background: Periodontitis results from the interaction between a subgingival biofilm and host immune response. Changes in biofilm composition are thought to disrupt homeostasis between the host and subgingival bacteria resulting in periodontal damage. Chronic systemic inflammatory disorders have been shown to affect the subgingival microbiota and clinical periodontal status. However, this relationship has not been examined in subjects with systemic lupus erythematosus (SLE). The objective of our study was to investigate the influence of SLE on the subgingival microbiota and its connection with periodontal disease and SLE activity. Methods: We evaluated 52 patients with SLE compared to 52 subjects without SLE (control group). Subjects were classified as without periodontitis and with periodontitis. Oral microbiota composition was assessed by amplifying the V4 region of 16S rRNA gene from subgingival dental plaque DNA extracts. These amplicons were examined by Illumina MiSeq sequencing. Results: SLE patients exhibited higher prevalence of periodontitis which occurred at a younger age compared to subjects of the control group. More severe forms of periodontitis were found in SLE subjects that had higher bacterial loads and decreased microbial diversity. Bacterial species frequently detected in periodontal disease were observed in higher proportions in SLE patients, even in periodontal healthy sites such as Fretibacterium, Prevotella nigrescens, and Selenomonas. Changes in the oral microbiota were linked to increased local inflammation, as demonstrated by higher concentrations of IL-6, IL-17, and IL-33 in SLE patients with periodontitis. Conclusions: SLE is associated with differences in the composition of the microbiota, independently of periodontal status.
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页数:13
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