Complex formation of the neuron-specific ELAV-like hu RNA-binding proteins

被引：58

作者：

Kasashima, K ^{[1
]}

Sakashita, E ^{[1
]}

Saito, K ^{[1
]}

Sakamoto, H ^{[1
]}

机构：

[1] Kobe Univ, Grad Sch Sci & Technol, Dept Biol, Nada Ku, Kobe, Hyogo 6578501, Japan

来源：

NUCLEIC ACIDS RESEARCH | 2002年 / 30卷 / 20期

基金：

日本学术振兴会;

关键词：

D O I：

10.1093/nar/gkf567

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hu proteins are RNA-binding proteins that are the vertebrate homologs of Drosophila ELAV, and are implicated in stabilization or enhanced translation of specific mRNAs with AU-rich elements (AREs) in the 3'-untranslated region. Here, using the yeast two-hybrid system, we show that neuron-specific Hu proteins can interact with themselves. Immuno precipitation assays demonstrated that the interaction between Hu proteins occurs in mammalian cells and is strongly enhanced in the presence of cellular RNA. Furthermore, using in situ chemical crosslinking assays, we found that HuD, one of the neuron-specific Hu proteins, multimerizes in cells. The crosslinked HuD multimers retain specific RNA-binding ability and can interact with additional Hu proteins. Consistent with this biochemical property, HuD showed granular distribution in two neurogenic cell lines. These results suggest that the RNA-bound form of HuD multimerizes cooperatively to form a specific granular structure that may serve as a site of post-transcriptional regulation of ARE-containing mRNAs.

引用

页码：4519 / 4526

页数：8

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