Regulation of GPR55 in rat white adipose tissue and serum LPI by nutritional status, gestation, gender and pituitary factors

被引：27

作者：

Imbernon, Monica ^{[1
,2
]}

Whyte, Lauren ^{[3
]}

Diaz-Arteaga, Adenis ^{[1
,2
]}

Russell, Wendy R. ^{[4
]}

Moreno, Natalia R. ^{[2
,5
]}

Vazquez, Maria J. ^{[1
,2
]}

Gonzalez, Carmen R. ^{[1
,2
]}

Diaz-Ruiz, Alberto ^{[2
,5
]}

Lopez, Miguel ^{[1
,2
]}

Malagon, Maria M. ^{[2
,5
]}

Ross, Ruth A. ^{[3
]}

Dieguez, Carlos ^{[1
,2
]}

Nogueiras, Ruben ^{[1
,2
]}

机构：

[1] Univ Santiago, CIMUS, Inst Invest Sanitaria, Dept Physiol, Santiago De Compostela 15782, Spain

[2] CIBER Fisiopatol Obesidad & Nutr CIBERobn, Santiago De Compostela 15706, Spain

[3] Univ Toronto, Dept Pharmacol & Toxicol, Toronto, ON M5S 1A8, Canada

[4] Univ Aberdeen, Rowett Inst Nutr & Hlth, Aberdeen AB21 9S8, Scotland

[5] Univ Cordoba, Hosp Univ Reina Sofia, Inst Maimonides Invest Biomed IMIBIC, Dept Cell Biol Physiol & Immunol, E-14004 Cordoba, Spain

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2014年 / 383卷 / 1-2期

关键词：

Endocannabinoids; GPR55; CBI; White adipose tissue; LPI; Nutritional status; CANNABINOID RECEPTOR GPR55; MESSENGER-RIBONUCLEIC-ACID; GROWTH-HORMONE; ENDOCANNABINOID SYSTEM; INSULIN-RESISTANCE; FOOD RESTRICTION; LEPTIN; METABOLISM; SUPPRESSION; SENSITIVITY;

D O I：

10.1016/j.mce.2013.12.011

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

The G protein-coupled receptor GPR55 has been proposed as a new cannabinoid receptor associated with obesity in humans. We have investigated the regulation of GPR55 in rat white adipose tissue (WAT) in different physiological and pathophysiological settings involved in energy balance. We compared GPR55 expression with Cannabinoid Receptor type I (CBI), which mediates the metabolic actions of endocannabinoids, by real time PCR and western blotting. Circulating levels of lysophosphatidylinositol (LPI), the endogenous ligand of GPR55, were measured by liquid chromatography-mass spectrometry. Both WAT CBI and GPR55 levels were increased after fasting and recovered after leptin treatment. Their expression was decreased during gestation and increased throughout lifespan. Orchidectomy diminished WAT CBI and GPR55 expression whereas ovariectomized rats showed increased GPR55 but decreased CBI levels. Alterations in pituitary functions also modified WAT CBI and GPR55 levels. Serum LPI levels were inversely regulated by fasting and gonadectomy in comparison to WAT GPR55. Our findings indicate that GPR55 and LPI are regulated by different physiological and pathophysiological settings known to be associated with marked alterations in energy status. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

引用

页码：159 / 169

页数：11

共 58 条

[1]

The orphan G protein-coupled receptor GPR55 promotes cancer cell proliferation via ERK [J].