Multiprotein immunoassay arrays fabricated by microcontact printing

被引：97

作者：

Inerowicz, HD

Howell, S

Regnier, FE

Reifenberger, R ^{[1
]}

机构：

[1] Purdue Univ, Dept Phys, W Lafayette, IN 47907 USA

[2] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

[3] Gdansk Tech Univ, Dept Chem, PL-80952 Gdansk, Poland

来源：

LANGMUIR | 2002年 / 18卷 / 13期

关键词：

D O I：

10.1021/la0157216

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Microarrays containing up to three different proteins were fabricated by microcontact printing (muCP) techniques and tested as a detection system for specific antibodies. After fabrication, immunoassays were successfully performed using the patterned protein microarrays. The developed immunoassays were characterized by fluorescence microscopy and scanning probe microscopy. The characterization revealed the quality of the protein deposition and indicated a high degree of selectivity for the targeted antigen-antibody interactions. The results of this study suggest that muCP is an inexpensive and effective way to fabricate biologically active substrates that can be of use for multiple reagentless immunosensor applications.

引用

页码：5263 / 5268

页数：6

共 14 条

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