High-performance liquid chromatography and inductively coupled plasma mass spectrometry (HPLC-ICP-MS) for the analysis of xenobiotic metabolites in rat urine: application to the metabolites of 4-bromoaniline

被引：37

作者：

Nicholson, JK

Lindon, JC

Scarfe, G

Wilson, ID

Abou-Shakra, F

Castro-Perez, J

Eaton, A

Preece, S

机构：

[1] AstraZeneca Pharmaceut, Drug Metab & Pharmacokinet, Macclesfield SK10 4TG, Cheshire, England

[2] Univ London Imperial Coll Sci Technol & Med, London SW7 2AZ, England

[3] Micromass, Manchester M23 9LZ, Lancs, England

来源：

ANALYST | 2000年 / 125卷 / 02期

关键词：

D O I：

10.1039/a909494f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of HPLC-ICP-MS for the profiling and quantification of the metabolites of 4-bromoaniline following reversed-phase gradient chromatography is demonstrated. In the 0-8 h post dose sample, which contained the highest concentrations of compound-related material, it was possible to detect at least 16 metabolites of the compound. The methodology described offers the possibility of obtaining metabolite profiles and quantification for drugs and other xenobiotics in biological fluids and excreta without the requirement for radiolabelled tracers.

引用

页码：235 / 236

页数：2

共 6 条

[1] Quantitative studies on the urinary metabolic fate of 2-chloro-4-trifluoromethylaniline in the rat using 19F-NMR spectroscopy and directly coupled HPLC-NMR-MS [J].