Rapid detection of the O25b-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strains

被引：315

作者：

Clermont, Olivier ^{[1
,2
]}

Dhanji, Hiran ^{[3
]}

Upton, Mathew ^{[4
]}

Gibreel, Tarek ^{[4
]}

Fox, Andrew ^{[5
]}

Boyd, David ^{[6
]}

Mulvey, Michael R. ^{[6
]}

Nordmann, Patrice ^{[7
]}

Ruppe, Etienne ^{[8
]}

Sarthou, Jean Louis ^{[8
]}

Frank, Thierry ^{[9
,10
]}

Vimont, Sophie ^{[9
,11
]}

Arlet, Guillaume ^{[9
,11
]}

Branger, Catherine ^{[1
,2
,12
]}

Woodford, Neil ^{[3
]}

Denamur, Erick ^{[1
,2
]}

机构：

[1] INSERM, U722, F-75018 Paris, France

[2] Univ Paris 07, Fac Med, F-75018 Paris, France

[3] Hlth Protect Agcy, Ctr Infect, Antibiot Resistance Monitoring & Reference Lab, London NW9 5HT, England

[4] Univ Manchester, Dept Med Microbiol, Sch Med, Manchester M13 9WL, Lancs, England

[5] Hlth Protect Agcy, North West Lab, Manchester M13 9WZ, Lancs, England

[6] Publ Hlth Agcy Canada, Natl Microbiol Lab, Antimicrobial Resistance & Nosocomial Infect, Winnipeg, MB, Canada

[7] Univ Paris Sud, Dept Bacteriol Virol, INSERM, U914,Hop Bicetre, F-94275 Le Kremlin Bicetre, France

[8] Inst Pasteur Cambodge, Phnom Penh, Cambodia

[9] Univ Paris 06, Fac Med, Dept Microbiol, UPRES EA2392, Paris, France

[10] Inst Pasteur, Bangui, Cent Afr Republ

[11] Hop Tenon, AP HP, Serv Bacteriol, F-75970 Paris, France

[12] Hop Louis Mourier, AP HP, Serv Microbiol Hyg, F-92701 Colombes, France

来源：

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 2009年 / 64卷 / 02期

关键词：

B2 phylogenetic subgroup I; ESBL; O25; ST; 131; DISSEMINATION; PREVALENCE; VIRULENCE; PARIS; AREA;

D O I：

10.1093/jac/dkp194

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Recently, a CTX-M-15 extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli O25b-ST131 clone, belonging to the B2 phylogenetic group and with a high virulence potential, has been reported all over the world, representing a major public health problem. The present study was carried out to develop a rapid and simple detection assay that identifies members of this clone. A total of 627 E. coli isolates of which 373 produced an ESBL, collected across four continents, were screened using a O25b-ST131 clone allele-specific PCR for the pabB gene. One hundred and forty-three ESBL isolates were found positive with the assay. These isolates were all of O25b type and, when studied by multilocus sequence typing (25 cases), were all of ST131. The O25b-ST131 clone was found to produce ESBLs other than CTX-M-15, specifically CTX-M-2, -3, -14, -27, -32 and -61 as well as TEM-24. This clone represents 3% of non-ESBL B2 isolates originating from urinary tract infections in Paris. We have developed a PCR-based assay that easily identifies a clone with high likelihood of producing ESBLs, including CTX-M-15.

引用

页码：274 / 277

页数：4

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