An immortalized goat mammary epithelial cell line induced with human telomerase reverse transcriptase (hTERT) gene transfer

被引：44

作者：

He, Y. L. ^{[1
]}

Wu, Y. H. ^{[1
]}

He, X. N. ^{[1
]}

Liu, F. J. ^{[2
]}

He, X. Y. ^{[1
]}

Zhang, Y. ^{[1
]}

机构：

[1] NW A&F Univ, Inst Biotechnol, Yangling 712100, Shaanxi, Peoples R China

[2] Henan Univ Sci & Technol, Coll Anim Sci & Technol, Luoyang 471003, Henan, Peoples R China

来源：

THERIOGENOLOGY | 2009年 / 71卷 / 09期

关键词：

Immortalization; Goat mammary epithelial cells; hTERT; Somatic cell nuclear transfer; Donor cells; NUCLEAR TRANSFER; LIFE-SPAN; FETAL; FIBROBLASTS; DIFFERENTIATION; EXPRESSION; EXTENSION; PASSAGES; OOCYTES;

D O I：

10.1016/j.theriogenology.2009.01.012

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Although mammary epithelial cell lines can provide a rapid and reliable indicator of gene expression efficiency of transgenic animals, their short lifespan greatly limits this application. To provide stable and long lifespan cells, goat mammary epithelial cells (GMECs) were transduced with pLNCX2-hTERT by retrovirus-mediated gene transfer. Transduced GMECs were evaluated by reverse transcriptase polymerase chain reaction (RT-PCR), proliferation assays, karyotype analysis, telomerase activity assay, western blotting, soft agar assay, and injection into nude mice. Non-transduced GMECs were used as a control. The hTERT-GMECs had higher telomerase activity and extended proliferative lifespan compared to non-transfected GMECs; even after Passage 50, hTERT-GMECs had a near diploid complement of chromosomes. Furthermore, they did not gain the anchroage-independent growth property and were not associated with a malignant phenotype in vitro or in vitro. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：1417 / 1424

页数：8

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