Structural heterogeneity of wheat arabinoxylans revealed by Raman spectroscopy

被引：31

作者：

Barron, Cecile

Robert, Paul

Guillon, Fabienne

Saulnier, Luc

Rouau, Xavier

机构：

[1] Univ Montpellier 2, ENSAM, INRA, CIRAD,UMR Ingn Agropolymeres & Technol Emergentes, F-34060 Montpellier, France

[2] INRA, UR Biopolymeres Interact & Assemblages, F-44316 Nantes, France

来源：

CARBOHYDRATE RESEARCH | 2006年 / 341卷 / 09期

关键词：

Raman spectroscopy; arabinoxylans; wheat;

D O I：

10.1016/j.carres.2006.03.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A set of arabinoxylan samples differing in their arabinose composition and various samples of arabino-xylo-oligosaccharide samples were analysed by Raman spectroscopy. Specific signatures for arabinose substitution were found in several spectral regions, that is, 400-600, 800-950 and 1030-1100 cm(-1). A linear relationship was observed between the peak ratio 855/895 cm(-1) of the second derivative spectra and the A/X ratio determined by chemical analysis. Moreover, spectral changes were observed in the 400-600 cm(-1) region assigned to the coupled vibrations mode in the skeleton: while the intensity of the band at 570 cm(-1) increased with the degree of substitution, that at 494 cm(-1) decreased. Similarly, a linear relationship was observed between the peak intensity ratio 570/494 cm(-1) calculated on the second derivative spectra and the composition data. Analysis of Raman spectra of arabino-xylo-oligosaccharides allowed to identify specific spectral features of disubstitution. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：1186 / 1191

页数：6

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