Determination by high-performance liquid chromatography of ketoprofen in vitro in rat skin permeation samples

被引：18

作者：

de Jalón, EG

Josa, M

Campanero, MA

Santoyo, S ^{[1
]}

Ygartua, P

机构：

[1] Univ Navarra, Ctr Galenico, Dept Farm & Tecnol Farmaceut, E-31080 Pamplona, Spain

[2] Univ Navarra Clin, Serv Farmacol Clin, E-31080 Pamplona, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 870卷 / 1-2期

关键词：

pharmaceutical analysis; ketoprofen; ibuprofen; nonsteroidal anti-inflammatory drugs;

D O I：

10.1016/S0021-9673(99)00995-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A direct, simple and rapid high-performance liquid chromatographic method has been developed for the determination of ketoprofen with ibuprofen as internal standard. Samples were chromatographed on a 5 mu m Kromasil 100 C-18 column. The mobile phase was a mixture of acetonitrile-0.01 M KH2PO4 adjusted to pH 1.5 with orthophosphoric acid 85% (60:40, v/v). Detection was at 260 nm and the run time was 10 min. The detector response was found to be linear in the concentration range 0.02 to 40 mu g/ml. This HPLC assay has been applied to measure the "in vitro" percutaneous penetration of ketoprofen through rat skin. (C) 2000 Elsevier Science BN. All rights reserved.

引用

页码：143 / 149

页数：7

共 17 条

[1]

The safety of topical anaesthetic and analgesic agents in a gel when used to provide pain relief at split skin donor sites [J].