In vivo enzymatic protein biotinylation

被引：65

作者：

Chapman-Smith, A

Cronan, JE

机构：

[1] Univ Illinois, Dept Microbiol, Chem & Life Sci Lab B103, Urbana, IL 61801 USA

[2] Univ Adelaide, Dept Biochem, Adelaide, SA 5005, Australia

[3] Univ Illinois, Dept Biochem, Chem & Life Sci Lab B103, Urbana, IL 61801 USA

来源：

BIOMOLECULAR ENGINEERING | 1999年 / 16卷 / 1-4期

关键词：

biotin; carboxylases and decarboxylases; biotinylation; protein modification; biotin ligase;

D O I：

10.1016/S1050-3862(99)00046-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Biotin is biologically active only when protein-bound and is covalently attached to a class of important metabolic enzymes, the biotin carboxylases and decarboxylases. Biotinylation is a relatively rare modification, with between one and five biotinylated protein species found in different organisms. We discuss the mechanism and structures involved in this extraordinarily specific protein modification and its exploitation in tagging recombinant proteins. (C) 1999 Published by Elsevier Science B.V. All rights reserved.

引用

页码：119 / 125

页数：7

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