Subtractive hybridization, a technique for extraction of DNA sequences distinguishing two closely related genomes: Critical analysis

被引：14

作者：

Ermolaeva, OD ^{[1
]}

Sverdlov, ED ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,SHEMYAKIN OVCHINNIKOV INST BIOORGAN CHEM,MOSCOW 117871,RUSSIA

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1996年 / 13卷 / 02期

关键词：

subtractive hybridization; enrichment; kinetics;

D O I：

10.1016/1050-3862(95)00152-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The present status of genomic DNA subtraction techniques is reviewed. The advantages and disadvantages of the widely-used methods of genome subtraction are discussed. Using the kinetic model of subtractive hybridization developed by us previously (Sverdlov and Ermolaeva, 1993; Sverdlov and Ermolaeva, 1994), the application of genome subtraction to various problems is analyzed. It is concluded that the technique should be further advanced based on subtraction of single-stranded DNAs. This strategy would enable one to efficiently extract target sequences omitting the stage of genome simplification.

引用

页码：49 / 58

页数：10

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