Purification, crystallization and preliminary X-ray diffraction analysis of yeast regulatory particle non-ATPase subunit 6 (Nas6p)

被引：4

作者：

Adachi, N

Padmanabhan, B

Kataoka, K

Kijima, K

Yamaki, M

Horikoshi, M

机构：

[1] Japan Sci & Technol Corp, ERATO, Horikoshi Gene Selector Project, Tsukuba, Ibaraki 3002635, Japan

[2] Univ Tokyo, Inst Mol & Cellular Biosci, Dev Biol Lab, Bunkyo Ku, Tokyo 1100032, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2002年 / 58卷

关键词：

D O I：

10.1107/S0907444902004808

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The regulatory particle non-ATPase subunit, Nas6p, from Saccharomyces cerevisiae has been crystallized by the hanging-drop vapour-diffusion method using PEG 4000 as precipitant. The crystals belong to the space group P2(1)2(1)2(1), with unit-cell parameters a = 41.43 (2), b = 61.74 (1), c = 98.09 (2) Angstrom, and contain one molecule in the asymmetric unit. A complete diffraction data set using synchrotron radiation was collected to 2.6 Angstrom resolution.

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页码：859 / 860

页数：2

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