Genetic differentiation between the clam species Ruditapes decussatus (grooved carpet shell) and Venerupis pullastra (pullet carpet shell) by PCR-SSCP analysis

被引：19

作者：

Fernández, A ^{[1
]}

García, T ^{[1
]}

Asensio, L ^{[1
]}

Rodríguez, MA ^{[1
]}

González, I ^{[1
]}

Lobo, E ^{[1
]}

Hernández, PE ^{[1
]}

Martín, R ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Vet, Dept Nutr & Bromatol 3, E-28040 Madrid, Spain

来源：

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 2002年 / 82卷 / 08期

关键词：

species identification; PCR-SSCP; clam species; Ruditapes decussatus; Venerupis pullastra;

D O I：

10.1002/jsfa.1117

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Genetic differentiation of the clam species Ruditapes decussatus (grooved carpet shell) and Venerupis pullastra (pullet carpet shell) has been achieved based on polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis. A short fragment (150 bp) of the alpha-actin gene was amplified by PCR. Amplicons were denatured to obtain single-stranded DNA, electrophoresed on a non-denaturing polyacrylamide gel and visualised by silver staining for detection of SSCPs. Species-specific DNA band patterns were obtained for R decussatus and V pullastra, allowing clear differentiation of the two clam species. (C) 2002 Society of Chemical Industry.

引用

页码：881 / 885

页数：5

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