Affinity chromatography with immunochemical detection applied to the analysis of human methionyl granulocyte colony stimulating factor in serum

被引：40

作者：

Miller, KJ

Herman, AC

机构：

[1] Amgen, Inc., Thousand Oaks, CA 91320-1789

来源：

ANALYTICAL CHEMISTRY | 1996年 / 68卷 / 18期

关键词：

D O I：

10.1021/ac960201e

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An on-line, automated, HPLC method was developed for the separation and detection of recombinant human methionyl granulocyte colony stimulating factor (GCSF) and GCSF modified with poly(ethylene glycol) (PEG-GCSF) in rat serum. The automated method consists of an initial immunoaffinity chromatography step, a microbore reverse phase separation, and postcolumn immunochemical detection. Calibration curves were constructed with a lower limit of 1.5 ng of GCSF (80 fmol) and 7.5 ng of PEG-GCSF in 120 mu L of rat serum, In vivo serum samples from rats dosed with PEG-GCSF were also analyzed. The development of the method is discussed as well as its general application to the detection of metabolites of recombinant proteins in body fluids.

引用

页码：3077 / 3082

页数：6

共 17 条

[1] AFFINITY PURIFICATION AND MICROCHARACTERIZATION OF RECOMBINANT DNA-DERIVED HUMAN GROWTH-HORMONE ISOLATED FROM AN IN-VIVO MODEL
BATTERSBY, JE
MUKKU, VR
CLARK, RG
HANCOCK, WS
[J]. ANALYTICAL CHEMISTRY, 1995, 67 (02) : 447 - 455
[2] PURIFICATION AND ANALYSIS OF DRUG RESIDUES IN URINE SAMPLES BY ONLINE IMMUNOAFFINITY CHROMATOGRAPHY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY CONTINUOUS-FLOW FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY
DAVOLI, E
FANELLI, R
BAGNATI, R
[J]. ANALYTICAL CHEMISTRY, 1993, 65 (19) : 2679 - 2685
[3] DELGADO C, 1992, CRIT REV THER DRUG, V9, P249
[4] EMNEUS J, 1995, J CHROMATOGR, V704, P191
[5] FERRAIOLO BL, 1992, PROTEIN PHARMACOKINE, pCH1
[6] Extracellular domain of granulocyte-colony stimulating factor receptor - Interaction with its ligand and identification of a domain in close proximity of ligand-binding region
Haniu, M
Horan, T
Arakawa, T
Le, J
Katta, V
Rohde, MF
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1995, 324 (02) : 344 - 356
[7] EVIDENCE FOR THE LIGAND-INDUCED CONVERSION FROM A DIMER TO A TETRAMER OF THE GRANULOCYTE-COLONY-STIMULATING FACTOR-RECEPTOR
HIRAOKA, O
ANAGUCHI, H
OTA, Y
[J]. FEBS LETTERS, 1994, 356 (2-3) : 255 - 260
[8] Dimerization of the extracellular domain of granulocyte-colony stimulating factor receptor by ligand binding: A monovalent ligand induces 2:2 complexes
Horan, T
Wen, J
Narhi, L
Parker, V
Garcia, A
Arakawa, T
Philo, J
[J]. BIOCHEMISTRY, 1996, 35 (15) : 4886 - 4896
[9] Automated analytical system for the examination of protein primary structure
Hsieh, YLF
Wang, HQ
Elicone, C
Mark, J
Martin, SA
Regnier, F
[J]. ANALYTICAL CHEMISTRY, 1996, 68 (03) : 455 - 462
[10] ICHIKAWA T, 1995, EXP HEMATOL, V23, P192

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