Terpene synthases from Cannabis sativa

被引：176

作者：

Booth, Judith K. ^{[1
]}

Page, Jonathan E. ^{[2
,3
]}

Bohlmann, Jorg ^{[1
,3
]}

机构：

[1] Univ British Columbia, Michael Smith Labs, Vancouver, BC V6T 1Z4, Canada

[2] Anandia Labs, Vancouver, BC V6T 1Z4, Canada

[3] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada

来源：

PLOS ONE | 2017年 / 12卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

CHAIN-LENGTH SPECIFICITY; GERANYLGERANYL DIPHOSPHATE; FUNCTIONAL EXPRESSION; GLANDULAR TRICHOMES; SMALL-SUBUNIT; BIOSYNTHESIS; EVOLUTION; GENERATION; PROTEINS; SYNERGY;

D O I：

10.1371/journal.pone.0173911

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cannabis (Cannabis sativa) plants produce and accumulate a terpene-rich resin in glandular trichomes, which are abundant on the surface of the female inflorescence. Bouquets of different monoterpenes and sesquiterpenes are important components of cannabis resin as they define some of the unique organoleptic properties and may also influence medicinal qualities of different cannabis strains and varieties. Transcriptome analysis of trichomes of the cannabis hemp variety `Finola' revealed sequences of all stages of terpene biosynthesis. Nine cannabis terpene synthases (CsTPS) were identified in subfamilies TPS-a and TPS-b. Functional characterization identified mono- and sesqui-TPS, whose products collectively comprise most of the terpenes of `Finola' resin, including major compounds such as P-myrcene, (E)-beta-ocimene, (-)-limonene, (+)-alpha-pinene, beta-caryophyllene, and alpha-humulene. Transcripts associated with terpene biosynthesis are highly expressed in trichomes compared to non-resin producing tissues. Knowledge of the CsTPS gene family may offer opportunities for selection and improvement of terpene profiles of interest in different cannabis strains and varieties.

引用

页数：20

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