Cooperativity and specificity of association of a designed transmembrane peptide

被引：24

作者：

Gratkowski, H

Dai, QH

Wand, AJ

DeGrado, WF

Lear, JD

机构：

[1] Johnson Fdn, Sch Med, Dept Biochem, Philadelphia, PA 19104 USA

[2] Univ Penn, Philadelphia, PA 19104 USA

来源：

BIOPHYSICAL JOURNAL | 2002年 / 83卷 / 03期

关键词：

D O I：

10.1016/S0006-3495(02)73930-1

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Thermodynamics studies aimed at quantitatively characterizing free energy effects of amino acid substitutions are not restricted to two state systems, but do require knowing the number of states involved in the equilibrium under consideration. Using analytical ultracentrifugation and NMR methods, we show here that a membrane-soluble peptide, MS1, designed by modifying the sequence of the water-soluble coiled-coil GCN4-P1, exhibits a reversible monomer-dimer-trimer association in detergent micelles with a greater degree of cooperativity in C14-betaine than in dodecyl phosphocholine detergents.

引用

收藏

页码：1613 / 1619

页数：7

相关论文

共 35 条

[1] Structure of the transmembrane cysteine residues in phospholamban [J].

Arkin, IT ;

Adams, PD ;

Brunger, AT ;

Aimoto, S ;

Engelman, DM ;

Smith, SO .

JOURNAL OF MEMBRANE BIOLOGY, 1997, 155 (03) :199-206

[2] A new data analysis method to determine binding constants of small molecules to proteins using equilibrium analytical ultracentrifugation with absorption optics [J].

Arkin, M ;

Lear, JD .

ANALYTICAL BIOCHEMISTRY, 2001, 299 (01) :98-107

[3] DESIGN OF 2-STRANDED AND 3-STRANDED COILED-COIL PEPTIDES [J].

BETZ, S ;

FAIRMAN, R ;

ONEIL, K ;

LEAR, J ;

DEGRADO, W .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1995, 348 (1323) :81-88

[4] Thermodynamic analysis of a designed three-stranded coiled coil [J].

Boice, JA ;

Dieckmann, GR ;

DeGrado, WF ;

Fairman, R .

BIOCHEMISTRY, 1996, 35 (46) :14480-14485

[5] Probing the folding and unfolding of wild-type and mutant forms of bacteriorhodopsin in micellar solutions: Evaluation of reversible unfolding conditions [J].

Chen, GQ ;

Gouaux, E .

BIOCHEMISTRY, 1999, 38 (46) :15380-15387

[6]

Choma C, 2000, NAT STRUCT BIOL, V7, P161

[7] Mutation and phosphorylation change the oligomeric structure of phospholamban in lipid bilayers [J].

Cornea, RL ;

Jones, LR ;

Autry, JM ;

Thomas, DD .

BIOCHEMISTRY, 1997, 36 (10) :2960-2967

[8]

EPSTEIN M, 1978, J BIOL CHEM, V253, P6660

[9] Detergents modulate dimerization but not helicity, of the glycophorin A transmembrane domain [J].

Fisher, LE ;

Engelman, DM ;

Sturgis, JN .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 293 (03) :639-651

[10] The effect of point mutations on the free energy of transmembrane alpha-helix dimerization [J].

Fleming, KG ;

Ackerman, AL ;

Engelman, DM .

JOURNAL OF MOLECULAR BIOLOGY, 1997, 272 (02) :266-275

← 1 2 3 4 →