α-amylase inactivation during corn starch hydrolysis process

被引:30
作者
Apar, DK [1 ]
Özbek, B [1 ]
机构
[1] Yildiz Tech Univ, Dept Chem Engn, TR-34210 Esenler, Turkey
关键词
corn starch hydrolysis; alpha-amylase inactivation; process variables; mathematical modelling; stirred batch bioreactor;
D O I
10.1016/j.procbio.2003.09.014
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effects of operating conditions on the enzymatic hydrolysis of corn starch were investigated. A commercial alpha-amylase produced by Bacillus sp. was used for the hydrolysis experiments. The degree of starch hydrolysis (%) and residual alpha-amylase activity (%) was investigated versus process variables, including pH, temperature, viscosity, impeller speed, processing time and some materials added such as hydrolysate, maltose, glucose, ethanol and CaCl2 using a stirred batch reactor. The mathematical models depending on the operating conditions were also derived using the experimental data of residual starch concentration. Some inactivation models were tested to determine the relationship between process variables and enzyme stability during the hydrolysis process. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1877 / 1892
页数:16
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