Small noncoding RNA GcvB is a novel regulator of acid resistance in Escherichia coli

被引：57

作者：

Jin, Ye ^{[1
]}

Watt, Rory M. ^{[2
]}

Danchin, Antoine ^{[3
]}

Huang, Jian-dong ^{[1
]}

机构：

[1] Univ Hong Kong, Dept Biochem, Li Ka Shing Fac Med, Pokfulam, Hong Kong, Peoples R China

[2] Univ Hong Kong, Fac Dent, Pokfulam, Hong Kong, Peoples R China

[3] Inst Pasteur, CNRS, URA2171, F-75724 Paris 15, France

来源：

BMC GENOMICS | 2009年 / 10卷

关键词：

DSRA RNA; SIGMA(S) SUBUNIT; RPOS TRANSLATION; BINDING-SITES; IN-VIVO; ACTIVATOR; GENE; EXPRESSION; PROTEIN; SYSTEM;

D O I：

10.1186/1471-2164-10-165

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: The low pH environment of the human stomach is lethal for most microorganisms; but not Escherichia coli, which can tolerate extreme acid stress. Acid resistance in E. coli is hierarchically controlled by numerous regulators among which are small noncoding RNAs (sncRNA). Results: In this study, we individually deleted seventy-nine sncRNA genes from the E. coli K12-MG1655 chromosome, and established a single-sncRNA gene knockout library. By systematically screening the sncRNA mutant library, we show that the sncRNA GcvB is a novel regulator of acid resistance in E. coli. We demonstrate that GcvB enhances the ability of E. coli to survive low pH by upregulating the levels of the alternate sigma factor RpoS. Conclusion: GcvB positively regulates acid resistance by affecting RpoS expression. These data advance our understanding of the sncRNA regulatory network involved in modulating acid resistance in E. coli.

引用

页数：7

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