Control with organic solvents of efficiency of persolvent cholesterol fermentation by Pseudomonas sp strain ST-200

被引：11

作者：

Doukyu, N ^{[1
]}

Kobayashi, H ^{[1
]}

Nakajima, H ^{[1
]}

Aono, R ^{[1
]}

机构：

[1] TOKYO INST TECHNOL, FAC BIOSCI & BIOTECHNOL, DEPT BIOENGN, YOKOHAMA, KANAGAWA 226, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 10期

关键词：

bioconversion of cholesterol; cholesterol conversion; Pseudomonas sp; persolvent fermentation; organic solvents;

D O I：

10.1271/bbb.60.1612

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pseudomonas sp. strain ST-200 oxidizes cholesterol dissolved in an organic solvent overlying the medium. Major conversion products are cholest-4-en-3-one (C4EO), 6 beta-hydroxcholest-4-en-3-one (HCEO), and cholest-4-ene-3,6-dione (CEDO). Productivity of each conversion product was altered by changing organic solvents used to dissolve the cholesterol. Generally, HCEO was predominant among the products. HCEO was produced even by cells grown without cholesterol and then killed with harmful organic solvents, The yield of the most oxidized product, CEDO, tvas improved when the cells; were grown in the presence of cholesterol dissolved in a less toxic solvent, cyclooctane.

引用

页码：1612 / 1616

页数：5

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