Cloning and sequencing of cDNAs encoding the human sperm protein, Sp17

被引：47

作者：

Lea, IA

Richardson, RT

Widgren, EE

ORand, MG

机构：

[1] Dept. of Cell Biology and Anatomy, Univ. of N. Carolina at Chapel Hill, CB #7090, Chapel Hill

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1307卷 / 03期

关键词：

cDNA cloning; spermatozoon; (human);

D O I：

10.1016/0167-4781(96)00077-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study we have cloned and sequenced two testis-specific cDNAs (1.3 kb and 1.6 kb) encoding a human sperm protein, designated HSp17. Each cDNA gave rise to identical protein sequences and differed only in the 5' untranslated region. The predicted amino-acid sequence revealed a protein of 17.5 kDa which exhibited a high degree of homology with both rabbit and mouse Sp17. Analysis of native and recombinant Sp17 by SDS-PAGE has shown the apparent molecular weight of the protein to be 24.5 kDa.

引用

页码：263 / 266

页数：4

共 4 条

[1] SEQUENCE AND LOCALIZATION OF THE MOUSE SPERM AUTOANTIGENIC PROTEIN, SP17
KONG, M
RICHARDSON, RT
WIDGREN, EE
ORAND, MG
[J]. BIOLOGY OF REPRODUCTION, 1995, 53 (03) : 579 - 590
[2] CHARACTERIZATION OF THE RABBIT SPERM MEMBRANE AUTO-ANTIGEN, RSA, AS A LECTIN-LIKE ZONA BINDING-PROTEIN
ORAND, MG
WIDGREN, EE
FISHER, SJ
[J]. DEVELOPMENTAL BIOLOGY, 1988, 129 (01) : 231 - 240
[3] SEQUENCE OF A RABBIT SPERM ZONA-PELLUCIDA BINDING-PROTEIN AND LOCALIZATION DURING THE ACROSOME REACTION
RICHARDSON, RT
YAMASAKI, N
ORAND, MG
[J]. DEVELOPMENTAL BIOLOGY, 1994, 165 (02) : 688 - 701
[4] TAYLOR SS, 1990, ANNU REV BIOCHEM, V59, P971, DOI 10.1146/annurev.bi.59.070190.004543

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