Differential induction of superoxide dismutase in downy mildew-resistant and -susceptible genotypes of pearl millet

被引：49

作者：

Babitha, MP

Bhat, SG

Prakash, HS ^{[1
]}

Shetty, HS

机构：

[1] Univ Mysore, Downy Mildew Res Lab, Dept Appl Bot & Biotechnol, Mysore 570006, Karnataka, India

[2] Cent Food Technol Res Inst, Dept Biochem & Nutr, Mysore 570013, Karnataka, India

来源：

PLANT PATHOLOGY | 2002年 / 51卷 / 04期

关键词：

downy mildew; host-pathogen interaction; isozymes; pearl millet; Sclerospora graminicola; superoxide dismutase;

D O I：

10.1046/j.1365-3059.2002.00733.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Differential induction of superoxide dismutase (SOD) in downy mildew-resistant and -susceptible genotypes of pearl millet (Pennisetum glaucum ) was observed on inoculation with Sclerospora graminicola . SOD activity was studied in resistant (IP18292) and susceptible (23B) pearl millet seedlings inoculated with S. graminicola . SOD activity increased by 2.3-fold in resistant seedlings upon inoculation. SOD activity was greatest in roots, with a specific activity of 3182 U per mg protein, after inoculation. SOD activity increased in all the resistant genotypes upon inoculation with S. graminicola . Native PAGE analysis showed four isozymes of SOD, three of which (SOD-1, -2 and -4) were Cu/Zn-SOD, whereas isozyme SOD-3 was Mn-SOD. This study also revealed increased intensity of all four isozymes of SOD in the resistant genotype upon inoculation. The involvement of SOD in pearl millet (host)-downy mildew pathogen interaction is discussed.

引用

页码：480 / 486

页数：7

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