Type IV carbonic anhydrase is present in the gills of spiny dogfish (Squalus acanthias)

被引:32
作者
Gilmour, K. M.
Bayaa, M.
Kenney, L.
McNeill, B.
Perry, S. F.
机构
[1] Univ Ottawa, Dept Biol, Ottawa, ON K1N 6N5, Canada
[2] Univ Ottawa, Ctr Adv Res Environm Genom, Ottawa, ON K1N 6N5, Canada
关键词
carbon dioxide excretion; gill;
D O I
10.1152/ajpregu.00477.2006
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Physiological and biochemical studies have provided indirect evidence for a membrane-associated carbonic anhydrase (CA) isoform, similar to mammalian type IV CA, in the gills of dogfish (Squalus acanthias). This CA isoform is linked to the plasma membrane of gill epithelial cells by a glycosylphosphatidylinositol anchor and oriented toward the plasma, such that it can catalyze the dehydration of plasma HCO3- ions. The present study directly tested the hypothesis that CA IV is present in dogfish gills in a location amenable to catalyzing plasma HCO3- dehydration. Homology cloning techniques were used to assemble a 1,127 base pair cDNA that coded for a deduced protein of 306 amino acids. Phylogenetic analysis suggested that this protein was a type IV CA. For purposes of comparison, a second cDNA (1,107 base pairs) was cloned from dogfish blood; it encoded a deduced protein of 260 amino acids that was identified as a cytosolic CA through phylogenetic analysis. Using real-time PCR and in situ hybridization, mRNA expression for the dogfish type IV CA was detected in gill tissue and specifically localized to pillar cells and branchial epithelial cells that flanked the pillar cells. Immunohistochemistry using a polyclonal antibody raised against rainbow trout type IV CA revealed a similar pattern of CA IV immunoreactivity and demonstrated a limited degree of colocalization with Na+-K(+)ATPase immunoreactivity. The presence and localization of a type IV CA isoform in the gills of dogfish is consistent with the hypothesis that branchial membrane-bound CA with an extracellular orientation contributes to CO2 excretion in dogfish by catalyzing the dehydration of plasma HCO3- ions.
引用
收藏
页码:R556 / R567
页数:12
相关论文
共 74 条
[1]   Catalysis and inhibition of human carbonic anhydrase IV [J].
Baird, TT ;
Waheed, A ;
Okuyama, T ;
Sly, WS ;
Fierke, CA .
BIOCHEMISTRY, 1997, 36 (09) :2669-2678
[2]   EFFECTS OF PERFUSATE BUFFER CAPACITY ON CAPILLARY CO2-HCO-3-H+ REACTIONS - THEORY [J].
BIDANI, A ;
HEMING, TA .
JOURNAL OF APPLIED PHYSIOLOGY, 1991, 71 (04) :1460-1468
[3]   ANALYSIS OF ABNORMALITIES OF CAPILLARY CO2 EXCHANGE INVIVO [J].
BIDANI, A .
JOURNAL OF APPLIED PHYSIOLOGY, 1991, 70 (04) :1686-1699
[4]   LOCALIZATION OF MEMBRANE-ASSOCIATED CARBONIC-ANHYDRASE TYPE-IV IN KIDNEY EPITHELIAL-CELLS [J].
BROWN, D ;
ZHU, XL ;
SLY, WS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (19) :7457-7461
[5]   Kinetics of CO2 excretion and intravascular pH disequilibria during carbonic anhydrase inhibition [J].
Cardenas, V ;
Heming, TA ;
Bidani, A .
JOURNAL OF APPLIED PHYSIOLOGY, 1998, 84 (02) :683-694
[6]   EFFECTS OF RED-BLOOD-CELL HCO3--CL EXCHANGE KINETICS ON LUNG CO2 TRANSFER - THEORY [J].
CRANDALL, ED ;
BIDANI, A .
JOURNAL OF APPLIED PHYSIOLOGY, 1981, 50 (02) :265-271
[7]  
ESBAUGH A, IN PRESS RESP PHYSL
[8]   Tribute to R.G.!Boutilier:: Evidence of a high activity carbonic anhydrase isozyme in the red blood cells of an ancient vertebrate, the sea lamprey Petromyzon marinus [J].
Esbaugh, AJ ;
Tufts, BL .
JOURNAL OF EXPERIMENTAL BIOLOGY, 2006, 209 (07) :1169-1178
[9]   Cytoplasmic carbonic anhydrase isozymes in rainbow trout Oncorhynchus mykiss:: comparative physiology and molecular evolution [J].
Esbaugh, AJ ;
Perry, SF ;
Bayaa, M ;
Georgalis, T ;
Nickerson, J ;
Tufts, BL ;
Gilmour, KM .
JOURNAL OF EXPERIMENTAL BIOLOGY, 2005, 208 (10) :1951-1961
[10]   Comparative physiology and molecular analysis of carbonic anhydrase from the red blood cells of teleost fish [J].
Esbaugh, AJ ;
Lund, SG ;
Tufts, BL .
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY, 2004, 174 (05) :429-438