Adipose tissue transcriptome by serial analysis of gene expression

被引:31
作者
Bolduc, C [1 ]
Larose, M [1 ]
Lafond, N [1 ]
Yoshioka, M [1 ]
Rodrigue, MA [1 ]
Morissette, J [1 ]
Labrie, C [1 ]
Raymond, V [1 ]
St-Amand, J [1 ]
机构
[1] Univ Laval, Med Ctr, CHUL,Lab SAGE, Quebec Genome Ctr,Mol Endocrinol & Oncol Res Ctr, Quebec City, PQ G1V 4G2, Canada
来源
OBESITY RESEARCH | 2004年 / 12卷 / 05期
关键词
SAGE; genetic engineering; transcriptome; functional genomic; bioinformatic;
D O I
10.1038/oby.2004.90
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: To describe the genomic expression profile or transcriptome of adipose tissue using the serial analysis of gene expression method. Research Methods and Procedures: The serial analysis of gene expression strategy is based on isolation of short sequences (tags), which usually correspond to unique transcripts, and on their concatenation into long DNA molecules, which are then cloned and sequenced. Experiments were performed with mRNA from retroperitoneal adipose tissue of male C57BL6 mice. Results: We isolated 45,996 tags corresponding to more than 17,000 different genes. Eighty-eight genes were expressed at more than 0.1 % of the total population and represented 26% of the mRNA population identified. The most expressed genes were: carbonic anhydrase 3 (1.97%), cytochrome c oxidase (COX) 1 (1.47%), COX2 (1.25%), diazepam binding inhibitor (1.04%), a novel transcript (0.87%), COX3 (0.55%), fatty acid-binding protein 4 (0.55%), and NADH dehydrogenase 4 (0.52%). Other genes known to be expressed in adipose tissue, such as uncoupling protein 2, angiotensinogen, adipsin, and insulin-like growth factor 1, were found at a lower level. Several tags corresponding to novel transcripts were also found. Discussion: To our knowledge, the present results provide for the first time a quantitative description of the transcriptome in adipose tissue.
引用
收藏
页码:750 / 757
页数:8
相关论文
共 19 条
[1]  
ADAMS MD, 1995, NATURE, V377, P3
[2]   EXPRESSION OF SPECIFIC MESSENGER-RNAS DURING ADIPOSE DIFFERENTIATION - IDENTIFICATION OF AN MESSENGER-RNA ENCODING A HOMOLOG OF MYELIN-P2 PROTEIN [J].
BERNLOHR, DA ;
ANGUS, CW ;
LANE, MD ;
BOLANOWSKI, MA ;
KELLY, TJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (17) :5468-5472
[3]  
Coe NR, 1999, J LIPID RES, V40, P967
[4]   SNAP23 promotes insulin-dependent glucose uptake in 3T3-L1 adipocytes: possible interaction with cytoskeleton [J].
Foster, LJ ;
Yaworsky, K ;
Trimble, WS ;
Klip, A .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1999, 276 (05) :C1108-C1114
[5]   Partial genome scale analysis of gene expression in human adipose tissue using DNA array [J].
Gabrielsson, BL ;
Carlsson, B ;
Carlsson, LMS .
OBESITY RESEARCH, 2000, 8 (05) :374-384
[6]   THE FUNCTION OF ACYL-COA-BINDING PROTEIN (ACBP)/DIAZEPAM BINDING INHIBITOR (DBI) [J].
KNUDSEN, J ;
MANDRUP, S ;
RASMUSSEN, JT ;
ANDREASEN, PH ;
POULSEN, F ;
KRISTIANSEN, K .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 1993, 123 (1-2) :129-138
[7]   CARBONIC ANHYDRASE-III IN OBESE ZUCKER RATS [J].
LYNCH, CJ ;
BRENNAN, WA ;
VARY, TC ;
CARTER, N ;
DODGSON, SJ .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 264 (04) :E621-E630
[8]   The expression of adipogenic genes is decreased in obesity and diabetes mellitus [J].
Nadler, ST ;
Stoehr, JP ;
Schueler, KL ;
Tanimoto, G ;
Yandell, BS ;
Attie, AD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (21) :11371-11376
[9]   AROMATASE-ACTIVITY AND CONCENTRATIONS OF CORTISOL, PROGESTERONE AND TESTOSTERONE IN BREAST AND ABDOMINAL ADIPOSE-TISSUE [J].
NEWTON, CJ ;
SAMUEL, DL ;
JAMES, VHT .
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1986, 24 (05) :1033-1039
[10]   Characterization of variability in large-scale gene expression data: Implications for study design [J].
Novak, JP ;
Sladek, R ;
Hudson, TJ .
GENOMICS, 2002, 79 (01) :104-113