Liquid chromatographic-mass spectrometric determination of genistin and daidzin in soybean food samples after accelerated solvent extraction with modified content of extraction cell

被引：51

作者：

Klejdus, B ^{[1
]}

Mikelová, R ^{[1
]}

Adam, V ^{[1
]}

Zehnálek, J ^{[1
]}

Vacek, J ^{[1
]}

Kizek, R ^{[1
]}

Kubán, V ^{[1
]}

机构：

[1] Mendel Univ Brno, Dept Chem & Biochem, CZ-61300 Brno, Czech Republic

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 517卷 / 1-2期

关键词：

accelerated solvent extraction; solvent extraction; ultrasonic extraction; Soxhlet extraction; high-performance liquid chromatography; UV spectrophotometric detection; mass spectrometry; isoflavones; daidzin; genistin; soybean food; foodstuffs;

D O I：

10.1016/j.aca.2004.05.003

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A newly elaborated accelerated solvent extraction (ASE) procedure allowed more accurate (<2%) and precise (<5-8% rel.) HPLC/UV/MS determination of isoflavones in different kinds of food made from soybean (products of ecological agriculture), i.e. soy farina, soy meat granulate, soy meat laminae, soy meat bits, Protmix and EKO-B1. The on-column limits of detection (LOD) for daidzin/genistin were 1.2/1.6 fmol for the signal-to-noise ratio S/N = 3 and 1-3 fmol for daidzem, genistein, formononetin, biochanin A and ononin, respectively. Final concentrations of daidzin in soybean foods fluctuated between 30 and 60 mug/g of dry matter (DM). Significantly higher concentrations of genistin (between 60 and 130 mug/g DM) were found in the soybean foods. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：1 / 11

页数：11

共 46 条

[1]

ADLERCREUTZ H, 1982, LANCET, V2, P1295

[2] Phyto-oestrogens and Western diseases [J].

Adlercreutz, H ;

Mazur, W .

ANNALS OF MEDICINE, 1997, 29 (02) :95-120

[3] Pressurized liquid extraction for the determination of polyphenols in apple [J].

Alonso-Salces, RM ;

Korta, E ;

Barranco, A ;

Berrueta, LA ;

Gallo, B ;

Vicente, F .

JOURNAL OF CHROMATOGRAPHY A, 2001, 933 (1-2) :37-43

[4] ORIGIN OF LIGNANS IN MAMMALS AND IDENTIFICATION OF A PRECURSOR FROM PLANTS [J].

AXELSON, M ;

SJOVALL, J ;

GUSTAFSSON, BE ;

SETCHELL, KDR .

NATURE, 1982, 298 (5875) :659-660

[5]

BAINES RN, 1999, ENV ANIMAL SAFETY DI, P45

[6] ISOFLAVONES AND THEIR CONJUGATES IN SOY FOODS - EXTRACTION CONDITIONS AND ANALYSIS BY HPLC MASS-SPECTROMETRY [J].

BARNES, S ;

KIRK, M ;

COWARD, L .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1994, 42 (11) :2466-2474

[7] Recent extraction techniques for solid matrices-supercritical fluid extraction, pressurized fluid extraction and microwave-assisted extraction: their potential and pitfalls [J].

Camel, V .

ANALYST, 2001, 126 (07) :1182-1193

[8] Extraction time for soybean isoflavone determination [J].

Carrao-Panizzi, MC ;

Favoni, SPG ;

Kikuchi, A .

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, 2002, 45 (04) :515-518

[9]

Chandra A, 1996, PHYTOCHEM ANALYSIS, V7, P259, DOI 10.1002/(SICI)1099-1565(199609)7:5<259::AID-PCA308>3.0.CO

[10]

2-S

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