Capillary electrophoretic separation of toxic drugs using a polyacrylamide-coated capillary

Capillary electrophoresis (CE) has recently become an attractive approach for the analysis of pharmaceuticals. In this study, capillary electrophoretic separation of anxiolytic drugs, including barbiturates and benzodiazepines, was carried out using polyacrylamide (PAA)-coated capillaries. The surface of the capillary inner wall was coated with a neutral layer, and separation was performed in the absence of electroosmotic flow (EOF). Both charged and neutral solutes were separated in the presence of sodium dodecyl sulfate (SDS) above its critical micelle concentration (CMC) in the running buffer. This kind of CE method provided fast and efficient separation of a total of 24 kinds of toxic drugs in a mixture. In addition, the analysis of toxic drugs in body fluids was attempted after the sample preparation using liquid-liquid extraction or solid-phase microextraction (SPME).