Method for assessment of functional affinity of antibodies for live bacteria

被引：14

作者：

Brovko, L ^{[1
]}

Young, D ^{[1
]}

Griffiths, MW ^{[1
]}

机构：

[1] Univ Guelph, Canadian Rs Inst Food Safety, Guelph, ON N1G 2W2, Canada

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2004年 / 58卷 / 01期

关键词：

functional affinity; antibodies; live bacteria;

D O I：

10.1016/j.mimet.2004.03.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and convenient method for assessment of functional affinity of antibodies against live bacteria is described. When a combination of immunomagnetic separation (IMS) with bioluminescent or fluorescent genetic labelling of the cells was employed, the method showed good correlation with plate count. However, the use of reporter bacteria allowed results to be obtained within 1 h compared with days using conventional methods. Due to its lower detection limit, the bioluminescent assay performed better than the fluorescent assay. Antibody affinities for Escherichia coli O157:H7 and Salmonella enteritidis were examined at different environmental conditions such as pH 3-7, temperature 4-25 degreesC, and sodium chloride concentrations 0-5% and compared with sensitivities of ELISA. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：49 / 57

页数：9

共 20 条

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