Stern-Volmer quenching and binding constants of 10-alkyl-9(10H)-acridone probes in SDS and BSA

Lucigenin (bis-N-methylacridinium nitrate) is an acridine-based probe that has been extensively used to detect reactive oxygen species (ROs) and has been shown to generate chemiluminescence in many biological materials. N-methylacridone is known to be the main emitter of the lucigenin's chemiluminescent system. This work investigates the distribution of synthesized 10-alkyl-9(10H)-acridone (-methyl, -butyl and -decyl) probes and quenchers (Cu2+ MV2+, BeV2+, Propy(+) and DoPy(+) in anionic micellar solution of sodium dodecyl sulfate (SDS). The binding ability of 10-alkyl-9(]OH)-acridone to a serum lipoprotein (BSA) was investigated. (C) 2002 Elsevier Science B.V. All rights reserved.