Sex differences in p-aminohippuric acid transport in rat kidney: Role of membrane fluidity and expression of OAT1

被引：50

作者：

Cerrutti, JA ^{[1
]}

Brandoni, A ^{[1
]}

Quaglia, NB ^{[1
]}

Torres, AM ^{[1
]}

机构：

[1] Univ Nacl Rosario, Fac Ciencias Bioquim & Farmaceut, RA-2000 Rosario, Santa Fe, Argentina

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2002年 / 233卷 / 1-2期

关键词：

organic anion transporter; p-aminohippuric acid; membrane fluidity; brush border membrane vesicles; basolateral membrane vesicles; renal tubular secretion;

D O I：

10.1023/A:1015563021602

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Sex differences in the kinetic parameters of p-aminohippuric acid (PAH) transport in brush border (BBMV) and basolateral (BLMV) membrane vesicles from kidney cortex have been observed. Membrane fluidity of BBMV was higher in females as compared with male rats as indicated by anisotropy values (0.1897 +/- 0.0010 vs. 0.2003 +/- 0.0014, p < 0.05, for females and males respectively). Membrane fluidity of BLMV were similar in both sexes. Western blot studies revealed that OAT1 protein in female BLMV was present at only 40% of level found in BLMV from male rats. The lower expression of OAT1 in BLMV in association with the higher BBMV fluidity (which may affect the affinity of PAH transporter in this membrane domain) observed in females may be responsible, at least in part, for the gender difference described in renal PAH secretion.

引用

页码：175 / 179

页数：5

共 26 条

[1] Regulation of renal tubular secretion of organic compounds [J].

Berkhin, EB ;

Humphreys, MH .

KIDNEY INTERNATIONAL, 2001, 59 (01) :17-30

[2]

BOWMAN HM, 1972, P SOC EXP BIOL MED, V141, P258

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4]

BRAUNLICH H, 1993, EXP TOXICOL PATHOL, V45, P309

[5]

BRENNER BB, 1996, KIDNEY, P607

[6] Structure of renal organic anion and cation transporters [J].

Burckhardt, G ;

Wolff, NA .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2000, 278 (06) :F853-F866

[7] Characterization of the mechanisms involved in the gender differences in p-aminohippurate renal elimination in rats [J].

Cerrutti, JA ;

Quaglia, NB ;

Torres, AM .

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 2001, 79 (09) :805-813

[8] Cellular and molecular aspects of drug transport in the kidney [J].

Inui, K ;

Masuda, S ;

Saito, H .

KIDNEY INTERNATIONAL, 2000, 58 (03) :944-958

[9]

JENSEN RE, 1988, J PHARMACOL EXP THER, V244, P543

[10] MEMBRANE FLUIDITY AND TRANSPORT-PROPERTIES IN EPITHELIA [J].

LEGRIMELLEC, C ;

FRIEDLANDER, G ;

ELYANDOUZI, E ;

ZLATKINE, P ;

GIOCONDI, MC .

KIDNEY INTERNATIONAL, 1992, 42 (04) :825-836

← 1 2 3 →