International Rice Genome Sequencing Project: the effort to completely sequence the rice genome

被引:333
作者
Sasaki, T
Burr, B
机构
[1] Natl Inst Agrobiol Resources, Rice Genome Res Program, Tsukuba, Ibaraki 3058602, Japan
[2] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA
关键词
D O I
10.1016/S1369-5266(99)00047-3
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The International Rice Genome Sequencing Project (IRGSP) involves researchers from ten countries who are working to completely and accurately sequence the rice genome within a short period. Sequencing uses a map-based clone-by-clone shotgun strategy; shared bacterial artificial chromosome/ P1-derived artificial chromosome libraries have been constructed from Oryra sativa ssp. japonica variety 'Nipponbare'. End-sequencing, fingerprinting and marker-aided PCR screening are being used to make sequence-ready contigs. Annotated sequences are immediately released for public use and are made available with supplemental information at each IRGSP member's website. The IRC;SP works to promote the development of rice and cereal genomics in addition to producing genome sequence data.
引用
收藏
页码:138 / 141
页数:4
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