Forced swimming differentially affects male and female brain corticosteroid receptors

被引:65
作者
Karandrea, D [1 ]
Kittas, C [1 ]
Kitraki, E [1 ]
机构
[1] Univ Athens, Sch Med, Lab Histol & Embryol, GR-11510 Athens, Greece
关键词
adrenal steroid receptors; sex dimorphism; stress; gonadal steroids; hippocampus; molecular neuroendocrinology;
D O I
10.1159/000054713
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Corticosteroid receptors are key mediators of the neuroendocrine response to stress. Previously, we have determined the effects of restraint stress on the regulation of corticosteroid receptor genes in the brain and pituitary of male and female rats. Significant gender- and regional-specific regulation of receptor mRNAs was observed. To further investigate the stressor specificity in the same context, we have determined glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) mRNAs following exposure to swimming stress paradigms applied alone, or in combination with restraint stress. Our data revealed stressor-specific alterations in GR or MR mRNA levels, which were more pronounced in males, the gender most affected by swimming stress. No alterations in GR or MR mRNA levels were detected in the female hippocampus and hypothalamus upon exposure to swimming paradigms, while in males the same stressors down-regulated GR mRNA in the hippocampus (chronic exposure) and up-regulated both genes in the hypothalamus (acute exposure). In the frontal cortex, acute swimming stress caused a reciprocal change in GR mRNA levels in the two sexes. The above difference is not due to circulating ovarian steroids, since ovariectomy did not change the female pattern of GR gene expression following acute stress. Our results further showed a hypothalamic-pituitary-adrenal axis facilitation to a novel superimposed stressor expressed at the level of limbic corticosteroid receptors: When chronically restrained rats of both sexes were exposed to acute swimming stress, a reduced GR/MR mRNA ratio, implying reduced feedback axis sensitivity, was detected in both the hippocampus and the hypothalamus. In conclusion, our work provides additional evidence on stressor, gender and region specificity in the regulation of brain corticosteroid receptors.
引用
收藏
页码:217 / 226
页数:10
相关论文
共 56 条
[1]   Corticotropin releasing hormone, receptor regulation and the stress response [J].
Aguilera, G .
TRENDS IN ENDOCRINOLOGY AND METABOLISM, 1998, 9 (08) :329-336
[2]  
ARIZZA J, 1988, NEURON, V1, P887
[3]   DISSOCIATION BETWEEN ADRENOCORTICOTROPIN AND CORTICOSTERONE RESPONSES TO RESTRAINT AFTER PREVIOUS CHRONIC EXPOSURE TO STRESS [J].
ARMARIO, A ;
RESTREPO, C ;
CASTELLANOS, JM ;
BALASCH, J .
LIFE SCIENCES, 1985, 36 (22) :2085-2092
[4]  
BODNOFF SR, 1995, J NEUROSCI, V15, P61
[5]   REPEATED MORPHINE ADMINISTRATION DOWN-REGULATES GLUCOCORTICOID, BUT NOT MINERALOCORTICOID, RECEPTORS IN THE RAT HIPPOCAMPUS [J].
BUDZISZEWSKA, B ;
JAWORSKA, L ;
LASON, W .
PSYCHONEUROENDOCRINOLOGY, 1995, 20 (01) :75-81
[6]   CHRONIC ESTROGEN-INDUCED ALTERATIONS IN ADRENOCORTICOTROPIN AND CORTICOSTERONE SECRETION, AND GLUCOCORTICOID RECEPTOR-MEDIATED FUNCTIONS IN FEMALE RATS [J].
BURGESS, LH ;
HANDA, RJ .
ENDOCRINOLOGY, 1992, 131 (03) :1261-1269
[7]   THE INFLUENCE OF OVARIAN-STEROIDS ON HYPOTHALAMIC-PITUITARY-ADRENAL REGULATION IN THE FEMALE RAT [J].
CAREY, MP ;
DETERD, CH ;
DEKONING, J ;
HELMERHORST, F ;
DEKLOET, ER .
JOURNAL OF ENDOCRINOLOGY, 1995, 144 (02) :311-321
[8]   REGULATION OF RAT MINERALOCORTICOID RECEPTOR EXPRESSION IN NEURONS BY PROGESTERONE [J].
CASTREN, M ;
PATCHEV, VK ;
ALMEIDA, OFX ;
HOLSBOER, F ;
TRAPP, T ;
CASTREN, E .
ENDOCRINOLOGY, 1995, 136 (09) :3800-3806
[9]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[10]   MODULATION OF HIPPOCAMPAL GLUCOCORTICOID AND MINERALOCORTICOID RECEPTOR MESSENGER-RNA EXPRESSION BY AMYGDALOID KINDLING [J].
CLARK, M ;
SMITH, MA ;
WEISS, SRB ;
POST, RM .
NEUROENDOCRINOLOGY, 1994, 59 (05) :451-456