SSR variation in important US maize inbred lines

被引:80
作者
Gethi, JG
Labate, JA
Lamkey, KR
Smith, ME
Kresovich, S
机构
[1] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA
[2] Cornell Univ, Dept Plant Breeding, Ithaca, NY 14853 USA
[3] Cornell Univ, USDA ARS, Plant Genet Resources Unit, Geneva, NY 14456 USA
[4] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Dept Agron, Ames, IA 50011 USA
关键词
D O I
10.2135/cropsci2002.0951
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Historically important public inbred lines continue to play an important role in maize (Zea mays L.) improvement in many different breeding programs. Their continued use means they have undergone numerous seed increases in diverse programs since their original release. Our objective was to estimate the level of genetic diversity among and within inbred lines from different sources using SSR markers. We sampled six inbred lines (B73. CM105. Mo17, Oh43, W153R, and Wf9) obtained from 14 sources (breeding programs). The data were analyzed by analysis of molecular variance (AMOVA), genetic diversity statistics, and genetic distance (Dice's coefficient). Of the total variation observed in gene frequency. 87.8% was found among inbred lines, 7.6% among sources within inbred lines, and 4.6% within sources. Genotypes of identically named inbred lines from eight different sources differed slightly on the basis of 44 SSR loci. The mean genetic similarity between sources of the same inbred was greater than 85%. It can be concluded that although more diversity exists among these six inbred lines than within them, a small but significant amount of variation exists among seed sources within inbreds. This variation may have arisen through differences in seed maintenance, since we found no evidence to suggest high mutation rates or extensive outcrossing. The small but statistically significant level of variation raises concerns in germplasm conservation, mapping studies, marker development', and long-term recombinant inbred line development, especially when high resolution is desired.
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页码:951 / 957
页数:7
相关论文
共 48 条
[1]   Genetic diversity and its relationship to hybrid performance in maize as revealed by RFLP and AFLP markers [J].
Ajmone Marsan P. ;
Castiglioni P. ;
Fusari F. ;
Kuiper M. ;
Motto M. .
Theoretical and Applied Genetics, 1998, 96 (2) :219-227
[2]  
*ARL, 2000, ARL US MAN VER 2 0 P
[3]  
BAKER R, 1984, P 20 ANN ILL CORN BR
[4]   GENETICS OF RESISTANCE IN MAIZE TO THE CORN LEAF APHID (HOMOPTERA, APHIDIDAE) [J].
BING, JW ;
GUTHRIE, WD ;
DICKE, FF .
JOURNAL OF ECONOMIC ENTOMOLOGY, 1992, 85 (04) :1476-1479
[5]   AN EVALUATION FOR GENETIC-VARIATION WITHIN MAIZE INBRED LINES MAINTAINED BY SIB-MATING AND SELF-POLLINATION [J].
BOGENSCHUTZ, TG ;
RUSSELL, WA .
EUPHYTICA, 1986, 35 (02) :403-412
[6]  
BUSCH ROBERT H., 1964, CROP SCI, V4, P400, DOI 10.2135/cropsci1964.0011183X000400040021x
[7]   1985 UNITED-STATES FARM MAIZE GERMPLASM BASE AND COMMERCIAL BREEDING STRATEGIES [J].
DARRAH, LL ;
ZUBER, MS .
CROP SCIENCE, 1986, 26 (06) :1109-1113
[8]   MEASURES OF THE AMOUNT OF ECOLOGIC ASSOCIATION BETWEEN SPECIES [J].
DICE, LR .
ECOLOGY, 1945, 26 (03) :297-302
[9]   Comparison of RFLP and morphological distances between maize Zea mays L inbred lines. Consequences for germplasm protection purposes [J].
Dillmann, C ;
BarHen, A ;
Guerin, D ;
Charcosset, A ;
Murigneux, A .
THEORETICAL AND APPLIED GENETICS, 1997, 95 (1-2) :92-102
[10]   Organization of RFLP diversity among inbred lines of maize representing the most significant heterotic groups [J].
Dubreuil, P ;
Dufour, P ;
Krejci, E ;
Causse, M ;
deVienne, D ;
Gallais, A ;
Charcosset, A .
CROP SCIENCE, 1996, 36 (03) :790-799