Effect of hypoxia on nitric oxide production and its synthase gene expression in rat smooth muscle cells

被引：17

作者：

Hong, Y

Suzuki, S ^{[1
]}

Yatoh, S

Mizutani, M

Nakajima, T

Bannai, S

Sato, H

Soma, M

Okuda, Y

Yamada, N

机构：

[1] Univ Tsukuba, Inst Clin Med, Dept Internal Med, Tsukuba, Ibaraki 305, Japan

[2] Univ Tokyo, Fac Med, Dept Internal Med 2, Tokyo, Japan

[3] Univ Tsukuba, Inst Basic Med Sci, Dept Biochem, Tsukuba, Ibaraki 305, Japan

[4] Mochida Pharmaceut Co Ltd, Pharmacovigilance Div, Tokyo, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 268卷 / 02期

关键词：

D O I：

10.1006/bbrc.2000.2140

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has not been clarified yet as to whether hypoxia and inflammation affect NO synthesis. In this study, we investigated the transcription of inducible nitric oxide synthase (iNOS) mRNA and the production of nitric oxide (NO) in rat smooth muscle cells (SMCs) cultured under hypoxic conditions in the presence and absence of proinflammatory cytokine interferon-gamma (IFN-gamma) and lipopolysaccharide (LPS), We found that hypoxia inhibited the production of NO but did not affect the transcription of iNOS mRNA in rat SMCs treated with IFN-gamma, LPS, or both, These results indicate that O-2 is involved in the regulation of NO synthesis in inflammatory tissues. (C) 2000 Academic Press.

引用

页码：329 / 332

页数：4

共 21 条

[1] LOSS OF ENDOTHELIUM-DEPENDENT RELAXANT ACTIVITY IN THE PULMONARY CIRCULATION OF RATS EXPOSED TO CHRONIC HYPOXIA [J].