Slik Sterile-20 kinase regulates Moesin activity to promote epithelial integrity during tissue growth

被引:74
作者
Hipfner, DR [1 ]
Keller, N [1 ]
Cohen, SM [1 ]
机构
[1] European Mol Biol Lab, D-69117 Heidelberg, Germany
关键词
Slik; Ste20; kinase; Moesin; epithelial integrity; apoptosis; actin cytoskeleton;
D O I
10.1101/gad.303304
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The Drosophila Sterile-20 kinase Slik promotes tissue growth during development by stimulating cell proliferation and by preventing apoptosis. Proliferation within an epithelial sheet requires dynamic control of cellular architecture. Epithelial integrity fails in slik mutant imaginal discs. Cells leave the epithelium and undergo apoptosis. The abnormal behavior of slik mutant cells is due to failure to phosphorylate and activate Moesin, which leads to excess Rho1 activity. This is distinct from Slik's effects on cell proliferation, which are mediated by Raf. Thus Slik acts via distinct pathways to coordinate cell proliferation with epithelial cell behavior during tissue growth.
引用
收藏
页码:2243 / 2248
页数:6
相关论文
共 37 条
[1]   Cooperative regulation of cell polarity and growth by Drosophila tumor suppressors [J].
Bilder, D ;
Li, M ;
Perrimon, N .
SCIENCE, 2000, 289 (5476) :113-116
[2]   The Ste20 group kinases as regulators of MAP kinase cascades [J].
Dan, I ;
Watanabe, NM ;
Kusumi, A .
TRENDS IN CELL BIOLOGY, 2001, 11 (05) :220-230
[3]   Direct binding of the Na-H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H+ translocation [J].
Denker, SP ;
Huang, DC ;
Orlowski, J ;
Furthmayr, H ;
Barber, DL .
MOLECULAR CELL, 2000, 6 (06) :1425-1436
[4]   Normal development of mice and unimpaired cell adhesion cell motility actin-based cytoskeleton without compensatory up-regulation of ezrin or radixin in moesin gene knockout [J].
Doi, Y ;
Itoh, M ;
Yonemura, S ;
Ishihara, S ;
Takano, H ;
Noda, T ;
Tsukita, S ;
Tsukita, S .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (04) :2315-2321
[5]   A matter of life and cell death [J].
Evan, G ;
Littlewood, T .
SCIENCE, 1998, 281 (5381) :1317-1322
[6]   EZRIN SELF-ASSOCIATION INVOLVES BINDING OF AN N-TERMINAL DOMAIN TO A NORMALLY MASKED C-TERMINAL DOMAIN THAT INCLUDES THE F-ACTIN BINDING-SITE [J].
GARY, R ;
BRETSCHER, A .
MOLECULAR BIOLOGY OF THE CELL, 1995, 6 (08) :1061-1075
[7]  
GATEFF E, 1978, SCIENCE, V200, P1448, DOI 10.1126/science.96525
[8]   Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway [J].
Gautreau, A ;
Poullet, PR ;
Louvard, D ;
Arpin, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (13) :7300-7305
[9]   The Drosophila Mst ortholog, hippo, restricts growth and cell proliferation and promotes apoptosis [J].
Harvey, KF ;
Pfleger, CM ;
Hariharan, IK .
CELL, 2003, 114 (04) :457-467
[10]   Association of ezrin with intercellular adhesion molecule-1 and -2 (ICAM-1 and ICAM-2) -: Regulation by phosphatidylinositol 4,5-bisphosphate [J].
Heiska, L ;
Alfthan, K ;
Grönholm, M ;
Vilja, P ;
Vaheri, A ;
Carpén, O .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (34) :21893-21900