Characterization of Leishmania sp strains isolated from autochthonous cases of human cutaneous leishmaniasis in Santa Catarina State, southern Brazil

被引：22

作者：

Grisard, EC ^{[1
]}

Steindel, M

Shaw, JJ

Ishikawa, EAY

Carvalho-Pinto, CJ

Eger-Mangrich, I

Toma, HK

Lima, JH

Romanha, AJ

Campbell, DA

机构：

[1] Univ Fed Santa Catarina, CCB, Dept Microbiol & Parasitol, Florianopolis, SC, Brazil

[2] FIOCRUZ, Ctr Pesquisas Rene Rachou, Lab Parasitol Celular & Mol, Belo Horizonte, MG, Brazil

[3] Univ Sao Paulo, ICB, Dept Parasitol, Sao Paulo, Brazil

[4] Univ Fed Rio de Janeiro, CCS, Dept Anal Clin & Toxicol, Rio De Janeiro, Brazil

[5] Univ Calif Los Angeles, Sch Med, Dept Microbiol & Immunol, Los Angeles, CA 90024 USA

[6] Inst Evandro Chagas FNS, Dept Parasitol, Belem, Para, Brazil

来源：

ACTA TROPICA | 2000年 / 74卷 / 01期

关键词：

human cutaneous leishmaniasis; Leishmania sp; mini-exon gene; spliced leader; characterization; monoclonal antibodies; PCR; hybridization;

D O I：

10.1016/S0001-706X(99)00054-6

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Four Leishmania sp. samples were isolated from autochthonous human cases of American cutaneous leishmaniasis (ACL) in Santa Catarina State, southern Brazil. These strains were characterized using indirect immunofluorescence with a panel of Leishmanin-specific monoclonal antibodies (MAbs), and by PCR amplification and hybridization assay of the mini-exon gene with group specific probes. The results obtained with the MAbs were in agreement with the genetic marker. Two isolates (MHOM/BR/89/JSC89-H1 and MHOMIBR/89/JSC89-H2) were identified as L. (Leishmania) amazonensis and two (MHOM/BR/96/LSC96-H3 and MHOM/BR/97/LSC97-H4) as L. (Viannia) braziliensis. The southernmost autochthonous cases of ACL in Brazil are due to two different Leishmania sp. species, confirming the spreading of ACL on the American continent. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：89 / 93

页数：5

共 10 条

[1] PCR detection and identification of Leishmania parasites in clinical specimens in Ecuador:: A comparison with classical diagnostic methods [J].

Aviles, H ;

Belli, A ;

Armijos, R ;

Monroy, FP ;

Harris, E .

JOURNAL OF PARASITOLOGY, 1999, 85 (02) :181-187

[2] MINI-EXON GENE VARIATION IN HUMAN PATHOGENIC LEISHMANIA SPECIES [J].

FERNANDES, O ;

MURTHY, VK ;

KURATH, U ;

DEGRAVE, WM ;

CAMPBELL, DA .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1994, 66 (02) :261-271

[3] IDENTIFICATION AND DISTRIBUTION OF NEW-WORLD LEISHMANIA SPECIES CHARACTERIZED BY SERODEME ANALYSIS USING MONOCLONAL-ANTIBODIES [J].

GRIMALDI, G ;

DAVID, JR ;

MCMAHONPRATT, D .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1987, 36 (02) :270-287

[4] Single-step multiplex PCR assay for characterization of New World Leishmania complexes [J].

Harris, E ;

Kropp, G ;

Belli, A ;

Rodriguez, B ;

Agabian, N .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (07) :1989-1995

[5]

McMahon-Pratt D., 1984, Genes and antigens of parasites. A laboratory manual. Proceedings of a course held 14 November-17 December 1983, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil., P121

[6] Zymodeme and serodeme characterization of Leishmania isolates obtained from Costa Rican patients [J].

Peraza, J ;

Urbina, A ;

Zeledon, R .

MEMORIAS DO INSTITUTO OSWALDO CRUZ, 1998, 93 (03) :283-287

[7] Detection and identification of human pathogenic Leishmania and Trypanosoma species by hybridization of PCR-amplified mini-exon repeats [J].

Ramos, A ;

Maslov, DA ;

Fernandes, O ;

Campbell, DA ;

Simpson, L .

EXPERIMENTAL PARASITOLOGY, 1996, 82 (03) :242-250

[8]

Sao Thiago PT, 1990, REV SOC BRAS MED TRO, V23, P201

[9] A RAPID AND SENSITIVE METHOD FOR THE IDENTIFICATION OF LEISHMANIA WITH MONOCLONAL-ANTIBODIES USING FLUORESCEIN-LABELED AVIDIN [J].

SHAW, JJ ;

ISHIKAWA, EAY ;

LAINSON, R .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1989, 83 (06) :783-784

[10]

*WHO, 1990, TECHN REP SER WHO, V793

← 1 →