The synthesis and evaluation of novel sialic acid analogues bound to matrices for the purification of sialic acid-recognising proteins

被引：13

作者：

Abo, S ^{[1
]}

Ciccotosto, S ^{[1
]}

Alafaci, A ^{[1
]}

von Itzstein, M ^{[1
]}

机构：

[1] Monash Univ, Dept Med Chem, Parkville, Vic 3052, Australia

来源：

CARBOHYDRATE RESEARCH | 1999年 / 322卷 / 3-4期

关键词：

sialic acid-recognising proteins; sialidases; thiosialosides; affinity chromatography;

D O I：

10.1016/S0008-6215(99)00249-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel N-acetylneuraminic acid analogue, 2-S-(5'-aminopentyl) 5-acetamido-3,5-dideoxy-2-thio-D-glycero-alpha-D-galacto-2-nonulopyranosidonic acid, as well as the thiosialoside 2-S-(2'-aminoethyl) 5-acetamido-3,5-dideoxy-2-thio-D-glycero-alpha-D-galacto-2-nonulopyranosidonic acid, have been synthesised and successfully coupled to CNBr-activated Sepharose 4B through the terminal amino group. The resultant affinity resins have proved efficient in purifying a number of sialic acid-recognising proteins such as Vibrio cholerae sialidase, sialidase-l from leech, trans-sialidase from Trypanosoma cruzi, and sialyltransferases from rat liver, all in high yield. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：201 / 208

页数：8

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