Orexin signaling in recombinant neuron-like cells

被引：59

作者：

Holmqvist, T

Åkerman, KEO

Kukkonen, JP

机构：

[1] Uppsala Univ, Dept Neurosci, BMC, SE-75123 Uppsala, Sweden

[2] AI Virtanen Inst Mol Sci, Kuopio, Finland

来源：

FEBS LETTERS | 2002年 / 526卷 / 1-3期

基金：

芬兰科学院;

关键词：

orexin; hypocretin; Ca2+; inositol phosphate; Ca2+ influx;

D O I：

10.1016/S0014-5793(02)03101-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To assess the role of orexin receptor signaling in neuron-like cells, Neuro-2a murine neuroblastoma and PC12 human pheochromocytoma cells were stably transfected with human OX1 or OX2 receptors. Activation of both receptors strongly elevated cellular inositol phosphates and Ca2+. A difference in the potency between orexin-A and -B was seen for OX1, but not OX2 receptors. Dependence of the orexin-mediated Ca2+ response on extracellular Ca2+ and the observed Ba2+ influx indicate that in addition to phospholipase C, orexin receptors also may couple to similar non-voltage-gated Ca2+ channels in neuronal cells as previously characterized in nonneuronal cells. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

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页码：11 / 14

页数：4

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