Microfracture and bone morphogenetic protein 7 (BMP-7) synergistically stimulate articular cartilage repair

被引:91
作者
Kuo, A. C.
Rodrigo, J. J.
Reddi, A. H.
Curtiss, S.
Grotkopp, E.
Chiu, M.
机构
[1] Univ Calif Davis, Dept Orthopaed Surg, Ctr Tissue Regenerat & Repair, Sacramento, CA 95817 USA
[2] Univ Calif Davis, Sect Evolut & Ecol, Davis, CA 95616 USA
[3] Univ Calif Davis, Dept Pathol, Davis, CA 95616 USA
关键词
microfracture; cartilage; bone morphogenetic protein 7; BMP-7;
D O I
10.1016/j.joca.2006.04.004
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Objective: Microfracture is used to treat articular cartilage injuries, but leads to the formation of fibrocartilage rather than native hyaline articular cartilage. Since bone morphogenetic protein 7 (BMP-7) induces cartilage differentiation, we hypothesized that the addition of the morphogen would improve the repair tissue generated by microfracture. We determined the effects of these two treatments alone and in combination on the quality and quantity of repair tissue formed in a model of full-thickness articular cartilage injury in adolescent rabbits. Design: Full-thickness defects were made in the articular cartilage of the patellar grooves of forty, 15-week-old rabbits. Eight animals were then assigned to (1) no further treatment (control), (2) microfracture, (3) BMP-7, (4) microfracture with BMP-7 in a collagen sponge (combination treatment), and (5) microfracture with a collagen sponge. Animals were sacrificed after 24 weeks at 39 weeks of age. The extent of healing was quantitated by determining the thickness and the surface area of the repair tissue. The quality of the repair tissue was determined by grading specimens using the International Cartilage Repair Society Visual Histological Assessment Scale. Results: Compared to controls, BMP-7 alone increased the amount of repair tissue without affecting the quality of repair tissue. Microfracture improved both the quantity and surface smoothness of repair tissue. Compared to either single treatment, the combination of microfracture and BMP-7 increased both the quality and quantity of repair tissue. Conclusions: Microfracture and BMP-7 act synergistically to stimulate cartilage repair, leading to larger amounts of repair tissue that more closely resembles native hyaline articular cartilage. (C) 2006 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
引用
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页码:1126 / 1135
页数:10
相关论文
共 28 条
[1]   HUMAN OSTEOGENIC PROTEIN-1 INDUCES BOTH CHONDROBLASTIC AND OSTEOBLASTIC DIFFERENTIATION OF OSTEOPROGENITOR CELLS DERIVED FROM NEWBORN RAT CALVARIA [J].
ASAHINA, I ;
SAMPATH, TK ;
NISHIMURA, I ;
HAUSCHKA, PV .
JOURNAL OF CELL BIOLOGY, 1993, 123 (04) :921-933
[2]   Human articular chondrocytes express osteogenic protein-1 [J].
Chubinskaya, S ;
Merrihew, C ;
Cs-Szabo, G ;
Mollenhauer, J ;
McCartney, J ;
Rueger, DC ;
Kuettner, KE .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2000, 48 (02) :239-250
[3]   Repair of articular cartilage defects with osteogenic protein-1 (BMP-7) in dogs [J].
Cook, SD ;
Patron, LP ;
Salkeld, SL ;
Rueger, DC .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2003, 85A :116-123
[4]   Arthroscopic subchondral bone plate microfracture technique augments healing of large chondral defects in the radial carpal bone and medial femoral condyle of horses [J].
Frisbie, DD ;
Trotter, GW ;
Powers, BE ;
Rodkey, WG ;
Steadman, JR ;
Howard, RD ;
Park, RD ;
McIlwraith, CW .
VETERINARY SURGERY, 1999, 28 (04) :242-255
[5]   OP-1 (BMP-7) affects mRNA expression of type 1, II, X collagen, and matrix Gla protein in ossifying long bones in vitro [J].
Haaijman, A ;
DSouza, RN ;
Bronckers, ALJJ ;
Goei, SW ;
Burger, EH .
JOURNAL OF BONE AND MINERAL RESEARCH, 1997, 12 (11) :1815-1823
[6]   Repair of partial-thickness defects in articular cartilage: Cell recruitment from the synovial membrane [J].
Hunziker, EB ;
Rosenberg, LC .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1996, 78A (05) :721-733
[7]   Growth-factor-induced healing of partial-thickness defects in adult articular cartilage [J].
Hunziker, EB .
OSTEOARTHRITIS AND CARTILAGE, 2001, 9 (01) :22-32
[8]   Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects [J].
Hunziker, EB .
OSTEOARTHRITIS AND CARTILAGE, 2002, 10 (06) :432-463
[9]   Regeneration of articular cartilage chondral defects by osteogenic protein-1 (bone morphogenetic protein-7) in sheep [J].
Jelic, M ;
Pecina, M ;
Haspl, M ;
Kos, J ;
Taylor, K ;
Maticic, D ;
McCartney, J ;
Yin, S ;
Rueger, D ;
Vukicevic, S .
GROWTH FACTORS, 2001, 19 (02) :101-113
[10]  
Klein-Nulend J, 1998, J BIOMED MATER RES, V40, P614, DOI 10.1002/(SICI)1097-4636(19980615)40:4<614::AID-JBM13>3.0.CO